Perils of gene mapping with microsatellite markers

The discovery of microsatellite polymorphisms has revitalized the genetic mapping of the human genome and promises to have a dramatic effect on human disease gene mapping. The high polymorphicity, relative abundance, and amenability of these markers to assay by PCR amplification gives them a signifi...

Full description

Saved in:
Bibliographic Details
Published in:American journal of human genetics 1992-10, Vol.51 (4), p.905-909
Main Authors: Knowles, J A, Vieland, V J, Gilliam, T C
Format: Article
Language:English
Subjects:
990300 > Information Handling
Alleles
BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
Chromosome Mapping
CHROMOSOMES
DATA BASE MANAGEMENT
DISEASES
DNA, Satellite - genetics
ENZYMES
Female
GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
GENETIC MAPPING
Genetic Markers
Genome, Human
Genotype
HUMAN CHROMOSOMES
Humans
Letter
Lod Score
Male
MANAGEMENT
MAPPING
MATHEMATICS
ORGANIC COMPOUNDS
Panic Disorder - genetics
Pedigree
Polymerase Chain Reaction
PROTEINS 550400 > Genetics
SAMPLING
STATISTICS
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The discovery of microsatellite polymorphisms has revitalized the genetic mapping of the human genome and promises to have a dramatic effect on human disease gene mapping. The high polymorphicity, relative abundance, and amenability of these markers to assay by PCR amplification gives them a significant advantage over previous markers, which explains their general acceptance and widespread use (Litt and Luty 1989; Weber and May 1989). Preliminary chromosome maps have been constructed using microsatellites exclusively (Weber et al. 1991; Hazen et al. 1992; Kwiatkowski et al. 1992), and disease loci have been mapped by linkage to these markers (Wijmenga et al. 1991). The markers provide new optimism for the mapping of disease genes, particularly for the mapping of complex genetic disorders. The authors present evidence that the very qualities that render these markers so efficient for chromosome mapping in large reference pedigrees can lead to dramatic lod score bias when applied to the typical pedigrees used to study genetic disorders, particularly when the disorder under study is complex. 11 refs., 2 figs., 1 tab.
ISSN:0002-9297
1537-6605