Transendothelial migration confers a survival advantage to activated T lymphocytes: role of LFA‐1/ICAM‐1 interactions

• Published in: Clinical and experimental immunology 2003-11, Vol.134 (2), p.246-252
• Main Authors: BORTHWICK, N. J., AKBAR, A. A., BUCKLEY, C., PILLING, D., SALMON, M., JEWELL, A. P., YONG, K. L.
• Format: Article
• Language: English
• Subjects: apoptosis; Apoptosis - immunology; Basic Immunology; Biological and medical sciences; Cell Line; Cell Movement - immunology; Cell Survival - immunology; Culture Media, Conditioned; Endothelium, Vascular - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; HUVEC; Immunopathology; Intercellular Adhesion Molecule-1 - immunology; Interferon-beta - immunology; Interleukin-2 - immunology; LFA‐1; Lymphocyte Activation - immunology; Lymphocyte Function-Associated Antigen-1 - immunology; Medical sciences; T lymphocytes; T-Lymphocytes - immunology
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1046/j.1365-2249.2003.02298.x

SUMMARY The clearance of activated T lymphocytes by apoptosis is an essential component in the resolution of the immune response; however, certain signals received within inflamed tissue may result in the persistence of activated T cells. Our previous work has shown that, when compared with resting cells, effector cells migrate more efficiently across endothelium, thus such cells may be selectively recruited to sites of inflammation. We hypothesized that transmigration of T cells across endothelium might influence cell survival. We have generated T cell lines by culturing in IL‐2 following PHA activation. These T cell lines die rapidly by apoptosis when deprived of IL‐2 (53·7 ± 4·0% survival after 24 h). In contrast, cells that have migrated across human umbilical vein endothelial cells (HUVEC) survived significantly better than control cells (80·3 ± 3·6%, n= 18, P