Loading…
Phospholemman expression is high in the newborn rabbit heart and declines with postnatal maturation
Phospholemman (PLM) is a small sarcolemmal protein that modulates the activities of Na +/K +-ATPase and the Na +/Ca 2+ exchanger (NCX), thus contributing to the maintenance of intracellular Na + and Ca 2+ homeostasis. We characterized the expression and subcellular localization of PLM, NCX, and the...
Saved in:
Published in: | Biochemical and biophysical research communications 2007-04, Vol.355 (2), p.338-341 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Phospholemman (PLM) is a small sarcolemmal protein that modulates the activities of Na
+/K
+-ATPase and the Na
+/Ca
2+ exchanger (NCX), thus contributing to the maintenance of intracellular Na
+ and Ca
2+ homeostasis. We characterized the expression and subcellular localization of PLM, NCX, and the Na
+/K
+-ATPase α1-subunit during perinatal development. Western blotting demonstrates that PLM (15
kDa), NCX (120
kDa), and Na
+/K
+-ATPase α-1 (∼100
kDa) proteins are all more than 2-fold higher in ventricular membrane fractions from newborn rabbit hearts (1–4-day old) compared to adult hearts. Our immunocytochemistry data demonstrate that PLM, NCX, and Na
+/K
+-ATPase are all expressed at the sarcolemma of newborn ventricular myocytes. Taken together, our data indicate that PLM, NCX, and Na
+/K
+-ATPase α-1 proteins have similar developmental expression patterns in rabbit ventricular myocardium. Thus, PLM may have an important regulatory role in maintaining cardiac Na
+ and Ca
2+ homeostasis during perinatal maturation. |
---|---|
ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2007.01.194 |