Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures

Using full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Fou...

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Bibliographic Details
Published in:Journal of clinical pathology 2006-02, Vol.59 (2), p.219-222
Main Authors: Lau, S K P, Ng, K H L, Woo, P C Y, Yip, K-t, Fung, A M Y, Woo, G K S, Chan, K-m, Que, T-l, Yuen, K-y
Format: Article
Language:English
Subjects:
16S rDNA gene
Accuracy
Adult
Aged
Aged, 80 and over
anaerobic Gram positive bacilli
Bacteria
Bacteria, Anaerobic - classification
Bacteria, Anaerobic - genetics
Bacteria, Anaerobic - isolation & purification
Bacterial Typing Techniques - methods
Biological and medical sciences
blood cultures
Deoxyribonucleic acid
Diagnostic Errors
DNA
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Female
Genes
Gram-Positive Bacteria - classification
Gram-Positive Bacteria - genetics
Gram-Positive Bacteria - isolation & purification
Gram-Positive Bacterial Infections - diagnosis
Gram-Positive Bacterial Infections - microbiology
Humans
Identification systems
Infant
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Microseq 500
Middle Aged
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Patients
Polymerase chain reaction
ribosomal RNA
RNA, Ribosomal, 16S - genetics
rRNA
Short Report
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Summary:Using full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.2004.025247