Morphine attenuates cholinergic nerve activity in human isolated colonic muscle

1 The action of morphine on cholinergic nerves in human sigmoid taenia coli muscle strips (taenia) was investigated using a radiolabelling technique. 2 Basal release of tritiated material from taenia was increased by electrical field stimulation (EFS). This increase was tetrodotoxin (3.14 μM)‐sensit...

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Bibliographic Details
Published in:British journal of pharmacology 1986-06, Vol.88 (2), p.307-313
Main Authors: Burleigh, David E., Trout, Stephen J.
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Colon - drug effects
Colon - innervation
Digestive system
Electric Stimulation
Exact sciences and technology
Hemicholinium 3 - pharmacology
Humans
In Vitro Techniques
Medical sciences
Morphine - pharmacology
Motor Neurons - drug effects
Muscle, Smooth - drug effects
Neurotransmitter Agents - metabolism
Other techniques and industries
Parasympathetic Nervous System - drug effects
Pharmacology. Drug treatments
Tetrodotoxin - pharmacology
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Summary:1 The action of morphine on cholinergic nerves in human sigmoid taenia coli muscle strips (taenia) was investigated using a radiolabelling technique. 2 Basal release of tritiated material from taenia was increased by electrical field stimulation (EFS). This increase was tetrodotoxin (3.14 μM)‐sensitive and calcium‐dependent. Analysis of basal and stimulated release of tritiated material indicated that evoked release (i.e. stimulated minus basal) is almost entirely due to an increase in [3H]‐acetylcholine ([3H]‐ACh) output. 3 Evoked release of [3H]‐ACh was dependent on the current strength and could be greatly reduced by exposing taenia to hemicholinium (34.8, 87.0 μM) before and during incubation with [3H]‐choline (4 μCi ml−1, 15 Ci mmol−1). 4 Spontaneous activity, muscle tone and the motor response of taenia to EFS were unaffected by morphine. 5 Evoked, but not basal, release of tritiated material was inhibited by morphine (1.32‐13.20 μM) in a concentration‐dependent manner. The inhibition of release was frequency‐dependent and naloxone (0.28 μM)‐sensitive. 6 The possible relationship between the effects of morphine on cholinergic nerves in taenia muscle and its actions in vivo are discussed.
ISSN:0007-1188
1476-5381
DOI:10.1111/j.1476-5381.1986.tb10206.x