Production of Adenovirus Antiserums in Horses

Studies were conducted to determine the suitability of using horses for the economical large-volume production of adenovirus antiserums. Antiserums were produced with adenovirus types 1 through 18. Methods of antigen production and control and immunization schedules are described. All serums were te...

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Bibliographic Details
Published in:Public health reports (1896) 1965-07, Vol.80 (7), p.647-652
Main Authors: James B. Lucas, J. Gibson Johnston, Jr, Harold S. Kaye, Matthew A. Bucca, Robinson, Roslyn Q.
Format: Article
Language:English
Subjects:
Adenoviridae
Adenoviridae Infections
Adenoviruses
Animals
Antigen-Antibody Reactions
Antigens
Antiserum
Blood
Erythrocytes
Hemagglutination
Hemagglutination Inhibition Tests
Horses
Immune Sera
Immunization Schedule
Neutralization Tests
Old Medline
Tissue culture techniques
Virus Cultivation
Viruses
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Summary:Studies were conducted to determine the suitability of using horses for the economical large-volume production of adenovirus antiserums. Antiserums were produced with adenovirus types 1 through 18. Methods of antigen production and control and immunization schedules are described. All serums were tested for homologous and heterotypic crosses in serum neutralization and hemagglutination inhibition tests. Most serums were found to be satisfactory for use as reference or working reagents for the identification of adenovirus isolates with either the serum neutralization or hemagglutination inhibition tests or both. Generally, serums were found to be less specific in the hemagglutination inhibition test, and greater variability with repeated testing was noted. The extent of heterologous cross reactivity among these 18 serotypes is reported, and suggestions are made that might minimize them in future production schedules. Studies with adenovirus types 3, 7a, and 14 disclosed that hemagglutination inhibition serum titers were not consistently reproducible with the same antigen preparation but different lots of rhesus erythrocytes. This variability in erythrocyte sensitivity causes a variation in antigen requirement for separate hemagglutination inhibition tests, which directly affects the titer obtained for the serum. For this reason it has been suggested that, with these three serotypes, a precise definition of serum titers should be expressed in terms of the hemagglutinin titration.
ISSN:0094-6214
DOI:10.2307/4592496