Critical role of stress in increased oesophageal mucosa permeability and dilated intercellular spaces

• Published in: Gut 2007-09, Vol.56 (9), p.1191-1197
• Main Authors: Farré, Ricard, De Vos, Rita, Geboes, Karel, Verbecke, Kristine, Berghe, Pieter Vanden, Depoortere, Inge, Blondeau, Kathleen, Tack, Jan, Sifrim, Daniel
• Format: Article
• Language: English
• Subjects: Acids; Animals; Biological and medical sciences; dilated intercellular spaces; DIS; Esophagus; Esophagus - drug effects; Esophagus - metabolism; Esophagus - ultrastructure; Experiments; Extracellular Space - drug effects; Gastro-Oesophageal Reflux; Gastroenterology. Liver. Pancreas. Abdomen; Gastroesophageal reflux; gastroesophageal reflux disease; Gastrointestinal Agents - pharmacology; GERD; HPA; Hydrochloric Acid - pharmacology; hypothalamic-pituitary-adrenal; Hypotheses; KHBB; Krebs–Henseleit bicarbonate buffer; Male; Mast Cells - cytology; Medical sciences; Microscopy, Electron - methods; Models, Animal; Mucous Membrane - drug effects; Mucous Membrane - metabolism; Mucous Membrane - ultrastructure; oesophageal mucosa; Other diseases. Semiology; Partial Restraint Stress; Pathogenesis; Pepsin A - pharmacology; Performance evaluation; Permeability; PRS; rat; Rats; Rats, Sprague-Dawley; Rodents; stress; Stress, Psychological - metabolism; Studies; TEM; transmission electron microscopy
• ISSN: 0017-5749; 1468-3288
• DOI: 10.1136/gut.2006.113688

Background: In patients with non-erosive gastroesophageal reflux disease, heartburn can occur when acid reaches sensory nerve endings through oesophageal-mucosa-dilated intercellular spaces. Stressful life events may increase heartburn perception. In the rat, acute stress increases gastric and intestinal mucosa permeability. We investigated whether acute stress can also increase oesophageal mucosa permeability and contribute to the dilation of mucosa intercellular spaces. Methods: Male Sprague–Dawley rats were submitted to partial restraint stress. Oesophageal mucosa from stressed and control rats was mounted in diffusion chambers. The permeability to 51Cr-EDTA (400 Da), fluorescein isothiocyanate (FITC)-dextran 4000 Da (FD4) and FITC-dextran 20 000 Da (FD20) was assessed after tissue incubation either with Krebs (control) or HCl pH 2.0+ pepsin 1 mg/ml. The diameter of intercellular spaces was assessed using transmission electron microscopy. Results: Acute stress increased faecal output, small-intestinal permeability and glycaemia. Exposure of oesophageal mucosa from control rats to acid-pepsin did not increase permeability to any of the tested molecules. Stress increased the number of submucosal mast cells and, by itself, increased the permeability to the smallest molecule (22.8±7.1 pmol/cm2 vs 5.8±2.1 pmol/cm2) (p