Survival of Salmonellae in Soft Agar

Two hundred glass ampules containing pure cultures of Salmonella in soft agar were sealed by melting and stored at intervals from 1929 to 1948. Eighty ampules contained Salmonella typhi; 60, Salmonella paratyphi A; and 60, Salmonella paratyphi B. The ampules were examined for surviving bacteria in 1...

Full description

Saved in:
Bibliographic Details
Published in:Public health reports (1896) 1970-09, Vol.85 (9), p.841-842
Main Authors: H. Kokolios, Ch. Paizis, F. Bredakis, Georgopoulos, A. P.
Format: Article
Language:English
Subjects:
Agar
Bacteria
Culture Media
Medical research
Melting
Microbiology
Salmonella
Salmonella - growth & development
Salmonella paratyphi A - growth & development
Salmonella typhimurium - growth & development
Time Factors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two hundred glass ampules containing pure cultures of Salmonella in soft agar were sealed by melting and stored at intervals from 1929 to 1948. Eighty ampules contained Salmonella typhi; 60, Salmonella paratyphi A; and 60, Salmonella paratyphi B. The ampules were examined for surviving bacteria in 1969. Interestingly, 151 (75 percent) of them contained viable salmonellae. The percentages of Salmonella surviving by culture were as follows: S. paratyphi B, 82 percent; S. paratyphi A, 77 percent; and S. typhi, 70 percent. The ampules last sealed had the greatest number of viable salmonellae. Eighty-two percent of the cultures in ampules sealed from 1941 to 1948 were viable, as were 78 percent of those sealed from 1934 to 1948 and 69 percent of those sealed from 1929 to 1933. These differences, however, were not statistically significant.
ISSN:0094-6214
DOI:10.2307/4593976