Maintaining the proper connection between the centrioles and the pericentriolar matrix requires Drosophila Centrosomin

Centrosomes consist of two centrioles surrounded by an amorphous pericentriolar matrix (PCM), but it is unknown how centrioles and PCM are connected. We show that the centrioles in Drosophila embryos that lack the centrosomal protein Centrosomin (Cnn) can recruit PCM components but cannot maintain a...

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Bibliographic Details
Published in:The Journal of cell biology 2007-08, Vol.178 (5), p.725-732
Main Authors: Lucas, Eliana P, Raff, Jordan W
Format: Article
Language:English
Subjects:
Animals
Brain - cytology
Cell division
Cell Division - physiology
Cells
Centrioles
Centrioles - metabolism
Centrioles - ultrastructure
Centrosome - chemistry
Centrosome - metabolism
Centrosomes
Cytoplasm - metabolism
Drosophila
Drosophila melanogaster - cytology
Drosophila melanogaster - embryology
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Embryos
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Humans
Insects
Larva - anatomy & histology
Larva - metabolism
Larvae
Microtubule-Organizing Center
Microtubules - metabolism
Mitosis
Mitotic spindle apparatus
Mutation
Neurons
Proteins
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Somatic cells
Stem cells
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Summary:Centrosomes consist of two centrioles surrounded by an amorphous pericentriolar matrix (PCM), but it is unknown how centrioles and PCM are connected. We show that the centrioles in Drosophila embryos that lack the centrosomal protein Centrosomin (Cnn) can recruit PCM components but cannot maintain a proper attachment to the PCM. As a result, the centrioles "rocket" around in the embryo and often lose their connection to the nucleus in interphase and to the spindle poles in mitosis. This leads to severe mitotic defects in embryos and to errors in centriole segregation in somatic cells. The Cnn-related protein CDK5RAP2 is linked to microcephaly in humans, but cnn mutant brains are of normal size, and we observe only subtle defects in the asymmetric divisions of mutant neuroblasts. We conclude that Cnn maintains the proper connection between the centrioles and the PCM; this connection is required for accurate centriole segregation in somatic cells but is not essential for the asymmetric division of neuroblasts.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200704081