ANDROGEN‐INDUCED SEXUAL DIMORPHISM IN HIGH AFFINITY DOPAMINE BINDING IN THE BRAIN TRANSCENDS THE HYPOTHALAMIC‐LIMBIC REGION

• Published in: British journal of pharmacology 1982-01, Vol.75 (1), p.37-48
• Main Authors: JALILIAN‐TEHRANI, MOHAMMAD H., KARAKIULAKIS, GEORGE, BLOND, CHRISTINE B., POWELL, RUTH, THOMAS, PATRICK J.
• Format: Article
• Language: English
• Subjects: Aging; Androgens - pharmacology; Animals; Binding Sites - drug effects; Brain - growth & development; Brain - metabolism; Castration; Dopamine - metabolism; Female; Male; Rats; Rats, Inbred Strains; Serotonin - metabolism; Sex Differentiation - drug effects; Sex Factors; Testosterone - pharmacology
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.1982.tb08755.x

1 High affinity binding of [3H]‐dopamine and [3H]‐5‐hydroxytryptamine ([3H]‐5‐HT) was measured in membrane fractions prepared from cerebral cortex, amygdala, hypothalamus, thalamus and brain stem of rats of either sex and of rats which had been either neonatally castrated or androgenized. 2 Binding was measured in rats of 8, 20 and 30 days old as well as in adults. 3 [3H]‐dopamine bound with approximately 30 nm affinity and [3H]‐5‐HT with approximately 10 nm affinity to all areas of the brain tested. The relative inhibitory effects of haloperidol, apomorphine, cis‐flupenthixol, unlabelled dopamine, noradrenaline, spiroperone, (+)‐butaclamol, fluphenazine, pimozide and 5‐HT on [3H]‐dopamine binding in the cerebral cortex was consistent with receptor status for the binding components there as were the relative inhibitory effects of methysergide, dopamine, fluoxetine and ouabain on [3H]‐5‐HT binding in the fore brain. 4 Neither [3H]‐dopamine nor [3H]‐5‐HT binding varied with the state of the sexual cycle in females. 5 There were no sexual differences in [3H]‐5‐HT binding in any of the brain areas tested nor was it affected by neonatal androgenization or neonatal castration. 6 [3H]‐dopamine binding was greater in the cerebral cortex and amygdala of male than of female rats. These differences could be mimicked artificially by neonatal castration of males (female type development) or neonatal androgenization of females (male type development). Sexual dimorphism did not become overt until 20 days of age and did not extend to hypothalamus, thalamus or brain stem. 7 It is concluded that neonatal sex differences in exposure to steroid hormones has permanent effects on the number of dopamine binding sites in the cerebral cortex and is suggested that this sexual dimorphism extends to the amygdala.