In vivo Monitoring of Ca2+Uptake into Mitochondria of Mouse Skeletal Muscle during Contraction

Although the importance of mitochondria in patho-physiology has become increasingly evident, it remains unclear whether these organelles play a role in Ca2+handling by skeletal muscle. This undefined situation is mainly due to technical limitations in measuring Ca2+transients reliably during the con...

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Bibliographic Details
Published in:The Journal of cell biology 2004-08, Vol.166 (4), p.527-536
Main Authors: Rudolf, Rüdiger, Mongillo, Marco, Magalhães, Paulo J., Pozzan, Tullio
Format: Article
Language:English
Subjects:
Calcium
Cellular biology
Fluorescence
Kinetics
Microscopy
Mitochondria
Muscle contraction
Muscle fibers
Muscles
Muscular system
Nervous system
Neurons
Rodents
Skeletal muscle
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Summary:Although the importance of mitochondria in patho-physiology has become increasingly evident, it remains unclear whether these organelles play a role in Ca2+handling by skeletal muscle. This undefined situation is mainly due to technical limitations in measuring Ca2+transients reliably during the contraction-relaxation cycle. Using two-photon microscopy and genetically expressed "cameleon" Ca2+sensors, we developed a robust system that enables the measurement of both cytoplasmic and mitochondrial Ca2+transients in vivo. We show here for the first time that, in vivo and under highly physiological conditions, mitochondria in mammalian skeletal muscle take up Ca2+during contraction induced by motor nerve stimulation and rapidly release it during relaxation. The mitochondrial Ca2+increase is delayed by a few milliseconds compared with the cytosolic Ca2+rise and occurs both during a single twitch and upon tetanic contraction.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200403102