Vacuolar Storage Proteins Are Sorted in the cis-Cisternae of the Pea Cotyledon Golgi Apparatus

Developing pea cotyledons contain functionally different vacuoles, a protein storage vacuole and a lytic vacuole. Lumenal as well as membrane proteins of the protein storage vacuole exit the Golgi apparatus in dense vesicles rather than in clathrin-coated vesicles (CCVs). Although the sorting recept...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 2001-01, Vol.152 (1), p.41-50
Main Authors: Hillmer, Stefan, Movafeghi, Ali, Robinson, David G., Hinz, Giselbert
Format: Article
Language:English
Subjects:
Antibodies
beta-Fructofuranosidase
Cellular biology
COP-Coated Vesicles - metabolism
Cotyledon - metabolism
Cotyledons
Glycoside Hydrolases - metabolism
Golgi apparatus
Golgi Apparatus - metabolism
High pressure
Legumins
Membrane Proteins - metabolism
Original
Peas
Pisum sativum
Plant cells
Plant Proteins - metabolism
Plants
Proteins
Receptors
Receptors, Cell Surface - metabolism
Seed Storage Proteins
Storage proteins
Vacuoles
Vacuoles - metabolism
Vesicular Transport Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Developing pea cotyledons contain functionally different vacuoles, a protein storage vacuole and a lytic vacuole. Lumenal as well as membrane proteins of the protein storage vacuole exit the Golgi apparatus in dense vesicles rather than in clathrin-coated vesicles (CCVs). Although the sorting receptor for vacuolar hydrolases BP-80 is present in CCVs, it is not detectable in dense vesicles. To localize these different vacuolar sorting events in the Golgi, we have compared the distribution of vacuolar storage proteins and of α-TIP, a membrane protein of the protein storage vacuole, with the distribution of the vacuolar sorting receptor BP-80 across the Golgi stack. Analysis of immunogold labeling from cryosections and from high pressure frozen samples has revealed a steep gradient in the distribution of the storage proteins within the Golgi stack. Intense labeling for storage proteins was registered for the cis-cisternae, contrasting with very low labeling for these antigens in the trans-cisternae. The distribution of BP-80 was the reverse, showing a peak in the trans-Golgi network with very low labeling of the cis-cisternae. These results indicate a spatial separation of different vacuolar sorting events in the Golgi apparatus of developing pea cotyledons.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.152.1.41