Oct4 dependence of chromatin structure within the extended Nanog locus in ES cells

Embryonic stem (ES) cells offer insight into early developmental fate decisions, and their controlled differentiation may yield vast regenerative potential. The molecular determinants supporting ES cell self-renewal are incompletely understood. The homeodomain proteins Nanog and Oct4 are essential f...

Full description

Saved in:
Bibliographic Details
Published in:Genes & development 2008-03, Vol.22 (5), p.575-580
Main Authors: Levasseur, Dana N, Wang, Jianlong, Dorschner, Michael O, Stamatoyannopoulos, John A, Orkin, Stuart H
Format: Article
Language:English
Subjects:
Animals
CCCTC-Binding Factor
Cell Differentiation - genetics
Cell Line
Chromatin - chemistry
Chromatin - metabolism
Chromatin - ultrastructure
Deoxyribonuclease I - chemistry
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Gene Expression Regulation, Developmental
Growth Differentiation Factor 3
Homeodomain Proteins - genetics
Insulator Elements
Intercellular Signaling Peptides and Proteins - genetics
Mice
Nanog Homeobox Protein
Octamer Transcription Factor-3 - genetics
Octamer Transcription Factor-3 - metabolism
Repressor Proteins - genetics
Repressor Proteins - metabolism
Research Communication
Transcription Factors - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Embryonic stem (ES) cells offer insight into early developmental fate decisions, and their controlled differentiation may yield vast regenerative potential. The molecular determinants supporting ES cell self-renewal are incompletely understood. The homeodomain proteins Nanog and Oct4 are essential for mouse ES cell self-renewal. Using a high-throughput approach, we discovered DNaseI hypersensitive sites and potential regulatory elements along a 160-kb region of the genome that includes GDF3, Dppa3, and Nanog. We analyzed gene expression, chromatin occupancy, and higher-order chromatin structure throughout this gene locus and found that expression of the reprogramming factor Oct4 is required to maintain its integrity.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1606308