A critical role for Cadherin6B in regulating avian neural crest emigration

Neural crest cells originate in the dorsal neural tube but subsequently undergo an epithelial-to-mesenchymal transition (EMT), delaminate, and migrate to diverse locations in the embryo where they contribute to a variety of derivatives. Cadherins are a family of cell–cell adhesion molecules expresse...

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Bibliographic Details
Published in:Developmental biology 2007-12, Vol.312 (2), p.533-544
Main Authors: Coles, E.G., Taneyhill, L.A., Bronner-Fraser, M.
Format: Article
Language:English
Subjects:
(E)migration
Animals
Avian Proteins - genetics
Avian Proteins - metabolism
Cadherin
Cadherins - genetics
Cadherins - metabolism
Cell adhesion
Cell Differentiation
Chick Embryo
Delamination
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
EMT
Epithelium - embryology
Epithelium - physiology
Gene Expression Regulation, Developmental
High Mobility Group Proteins - genetics
High Mobility Group Proteins - metabolism
Mesoderm - embryology
Mesoderm - physiology
Neural crest
Neural Crest - cytology
Neural Crest - embryology
Neural Crest - metabolism
SOXE Transcription Factors
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Neural crest cells originate in the dorsal neural tube but subsequently undergo an epithelial-to-mesenchymal transition (EMT), delaminate, and migrate to diverse locations in the embryo where they contribute to a variety of derivatives. Cadherins are a family of cell–cell adhesion molecules expressed in a broad range of embryonic tissues, including the neural tube. In particular, cadherin6B ( Cad6B) is expressed in the dorsal neural tube prior to neural crest emigration but is then repressed by the transcription factor Snail2, expressed by premigratory and early migrating cranial neural crest cells. To examine the role of Cad6B during neural crest EMT, we have perturbed Cad6B protein levels in the cranial neural crest-forming region and have examined subsequent effects on emigration and migration. The results show that knock-down of Cad6B leads to premature neural crest cell emigration, whereas Cad6B overexpression disrupts migration. Our data reveal a novel role for Cad6B in controlling the proper timing of neural crest emigration and delamination from the neural tube of the avian embryo.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2007.09.056