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JE Nakayama/JE SA-14-14-2 virus structural region intertypic viruses: Biological properties in the mouse model of neuroinvasive disease
A molecular clone of Japanese encephalitis (JE) virus Nakayama strain was used to create intertypic viruses containing either the 5’-C-prM-E or the prM-E region of the attenuated JE SA14-14-2 virus in the JE-Nakayama background. These two intertypic JE viruses, JE-X/5’CprME(S) and JE-X/prME(S) respe...
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Published in: | Virology (New York, N.Y.) N.Y.), 2007-05, Vol.366 (1), p.51-61 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | A molecular clone of Japanese encephalitis (JE) virus Nakayama strain was used to create intertypic viruses containing either the 5’-C-prM-E or the prM-E region of the attenuated JE SA14-14-2 virus in the JE-Nakayama background. These two intertypic JE viruses, JE-X/5’CprME(S) and JE-X/prME(S) respectively, generally resembled the parental JE virus in cell culture properties. Similar to virus derived from the JE Nakayama molecular clone (JE-XJN), JE-X/prME(S) was highly neuroinvasive and neurovirulent for young adult mice, whereas JE-X/5’CprME(S) was attenuated for neuroinvasiveness and only partially attenuated for neurovirulence. Immunization of young mice with JE-X/5’CprME(S) virus elicited neutralizing antibodies against JE Nakayama virus and conferred protection against encephalitis following challenge with JE Nakayama virus. The sequence of the JE-X/5’CprME(S) virus differed from that of JE-X/prME(S) virus at two nucleotides in the 5’ UTR, 3 amino acid positions in the capsid protein, 4 positions in the prM protein and 1 in the envelope protein. For JE-X/prME(S) virus, the 4 differences in prM and the single substitution in the envelope represented reversions to the sequence of JE-Nakayama virus. Overall, this study reveals that molecular determinants associated with the prM-E region of the attenuated JE SA14-14-2 virus are insufficient by themselves to confer an attenuation phenotype upon JE Nakayama virus. This suggests a role for determinants in the 5’ UTR and/or the capsid protein of the JE SA 14-14-2 virus genome in influencing the virulence properties of the JE Nakayama virus in the mouse model. |
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ISSN: | 0042-6822 1096-0341 |
DOI: | 10.1016/j.virol.2007.04.016 |