Influence of probiotic Lactobacilli colonization on neonatal B cell responses in a gnotobiotic pig model of human rotavirus infection and disease

• Published in: Veterinary immunology and immunopathology 2008-03, Vol.122 (1), p.175-181
• Main Authors: Zhang, Wei, Azevedo, Marli S.P., Gonzalez, Ana M., Saif, Linda J., Van Nguyen, Trang, Wen, Ke, Yousef, Ahmed E., Yuan, Lijuan
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Antibodies, Viral - blood; B cell responses; B-Lymphocytes - immunology; Disease Models, Animal; Gnotobiotic pigs; Human rotavirus; Lactobacillus; Lactobacillus acidophilus; Lactobacillus acidophilus - physiology; Lactobacillus reuteri - physiology; Probiotic; Probiotics - pharmacology; Rotavirus; Rotavirus Infections - immunology; Rotavirus Infections - virology; Swine; Virus Shedding
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2007.10.003

The goal of this study was to define the impact of colonization of gnotobiotic (Gn) pigs with lactic acid bacteria (LAB) on development of intestinal and systemic B cell responses to human rotavirus (HRV). The LAB-specific and total B cell responses were also assessed. Gn pigs were inoculated with LAB ( Lactobacillus acidophilus and L. reuteri) and virulent Wa strain HRV (LAB+HRV+), HRV only (LAB−HRV+), LAB only (LAB+HRV−) or mock (LAB−HRV−). The HRV infection induced similar HRV-specific intestinal and systemic antibody and B cell responses in pigs with or without LAB, whereas LAB significantly enhanced total intestinal IgA secreting cell responses and total serum IgM and intestinal IgM and IgG titers. The LAB colonization did not reduce HRV shedding or diarrhea, this may be partly due to the short time interval between the first LAB feeding and HRV inoculation. Further studies are needed with longer time for LAB to establish before HRV inoculation. However, our studies demonstrate that Gn pigs infected with HRV develop a similar magnitude of virus-specific B cell responses as those of HRV-infected and LAB colonized pigs. LAB colonization alone is not as efficient in promoting intestinal B cell responses, as is HRV infection.