Late Memory-Related Genes in the Hippocampus Revealed by RNA Fingerprinting

Although long-term memory is thought to require a cellular program of gene expression and increased protein synthesis, the identity of proteins critical for associative memory is largely unknown. We used RNA fingerprinting to identify candidate memory-related genes (MRGs), which were up-regulated in...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1997-09, Vol.94 (18), p.9669-9673
Main Authors: Cavallaro, Sebastiano, Meiri, Noam, Yi, Chu-Li, Musco, Simone, Ma, Wu, Goldberg, Jesse, Alkon, Daniel L.
Format: Article
Language:English
Subjects:
Animals
Behavioral neuroscience
Biological Sciences
Brain
Cellular biology
Complementary DNA
DNA fingerprints
Genes
Genetic Techniques
Hippocampus
Hippocampus - physiology
Male
Mazes
Memory
Memory - physiology
Messenger RNA
Molecular Sequence Data
Nerve Tissue Proteins - genetics
Polymerase chain reaction
Rats
Rats, Wistar
Ribonucleic acid
RNA
RNA - analysis
RNA - genetics
Swimming
Swimming - physiology
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Summary:Although long-term memory is thought to require a cellular program of gene expression and increased protein synthesis, the identity of proteins critical for associative memory is largely unknown. We used RNA fingerprinting to identify candidate memory-related genes (MRGs), which were up-regulated in the hippocampus of water maze-trained rats, a brain area that is critically involved in spatial learning. Two of the original 10 candidate genes implicated by RNA fingerprinting, the rat homolog of the ryanodine receptor type-2 and glutamate dehydrogenase (EC 1.4.1.3), were further investigated by Northern blot analysis, reverse transcription-PCR, and in situ hybridization and confirmed as MRGs with distinct temporal and regional expression. Successive RNA screening as illustrated here may help to reveal a spectrum of MRGs as they appear in distinct domains of memory storage.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.94.18.9669