The constitutive level of vascular endothelial growth factor (VEGF) is more important than hypoxia-induced VEGF up-regulation in the angiogenesis of human melanoma xenografts

Angiogenesis of tumours might develop as a result of environmental conditions, such as hypoxia, and/or as a result of genetic alterations specific for tumour cells. The relative contributions of these mechanisms were investigated by comparing the in vivo expression of vascular endothelial growth fac...

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Bibliographic Details
Published in:British journal of cancer 2000-05, Vol.82 (9), p.1528-1534
Main Authors: Danielsen, T, Rofstad, E K
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
Cell Hypoxia
Drug Resistance
Endothelial Growth Factors - metabolism
Epidemiology
Fibroblasts
General aspects (metabolism, cell proliferation, established cell line...)
Host-tumor relations. Immunology. Biological markers
Humans
Hypoxia
Immunohistochemistry
Lymphokines - metabolism
Medical research
Medical sciences
Melanoma
Melanoma - blood supply
Mice
Mice, Inbred BALB C
Molecular Medicine
Neoplasm Transplantation
Neovascularization, Pathologic
Oncology
Physiology
Regular
regular-article
Tumor cell
Tumors
Up-Regulation
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
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Summary:Angiogenesis of tumours might develop as a result of environmental conditions, such as hypoxia, and/or as a result of genetic alterations specific for tumour cells. The relative contributions of these mechanisms were investigated by comparing the in vivo expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) to the hypoxic fraction, the angiogenic potential and the vascular density of four human melanoma lines (A-07, D-12, R-18, U-25) grown intradermally in Balb/c nu/nu mice. VEGF expression, bFGF expression and expression of pimonidazole, a marker of hypoxic cells, were investigated by immunohistochemistry. An association between high VEGF and bFGF expression and high angiogenic potential was detected, suggesting an important role for VEGF/bFGF in the angiogenesis of melanomas. High VEGF/bFGF expression was also related to low hypoxic fraction and high vascular density. Thus, the constitutive, genetically determined level of VEGF was probably more important than hypoxia-induced upregulation in the angiogenesis of the melanoma xenografts. © 2000 Cancer Research Campaign
ISSN:0007-0920
1532-1827
DOI:10.1054/bjoc.2000.1173