Expression of the normal epithelial cell-specific 1 ( ; KLK10) candidate tumour suppressor gene in normal and malignant testicular tissue

The normal epithelial cell-specific 1 ( NES1 ) gene (official name kallikrein gene 10; KLK10) is a new member of the expanding human kallikrein gene family and encodes for a secreted serine protease. Experimental evidence suggests that NES1 controls normal cell growth and may function as a tumour su...

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Bibliographic Details
Published in:British journal of cancer 2001-07, Vol.85 (2), p.220-224
Main Authors: Luo, L-Y, Meyts, E Rajpert-De, Jung, K, Diamandis, E P
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Breast cancer
Cancer Research
DNA Primers
Drug Resistance
Epidemiology
Gene expression
Genes, Tumor Suppressor
Gynecology. Andrology. Obstetrics
Humans
Immunohistochemistry
Kallikreins - genetics
Male
Male genital diseases
Medical sciences
Molecular Medicine
Oncology
Polymerase chain reaction
Prostate cancer
Regular
regular-article
Reverse Transcriptase Polymerase Chain Reaction
RNA - metabolism
RNA, Neoplasm - metabolism
Testicular cancer
Testicular Neoplasms - genetics
Testis - metabolism
Tumors
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Summary:The normal epithelial cell-specific 1 ( NES1 ) gene (official name kallikrein gene 10; KLK10) is a new member of the expanding human kallikrein gene family and encodes for a secreted serine protease. Experimental evidence suggests that NES1 controls normal cell growth and may function as a tumour suppressor. NES1 is down-regulated during breast cancer progression. The NES1 gene is highly expressed in testicular as well as in other tissues. In this study, we investigated the expression level of the NES1 gene in cancerous and normal testicular tissues with reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. In all 14 primary testicular germ-cell tumours examined, the NES1 gene expression was markedly reduced compared to adjacent (paired) normal tissues. We further examined 6 randomly selected primary germ-cell tumours and 8 normal tissues (obtained from different individuals). We confirmed the differential expression of the NES1 gene in germ-cell tumours (GCT) and pre-malignant carcinoma in situ (CIS). Our findings suggest that NES1 may act as a tumour suppressor and may play a role in the pathogenesis and progression of this malignancy. © 2001 Cancer Research Campaign www.bjcancer.com
ISSN:0007-0920
1532-1827
DOI:10.1054/bjoc.2001.1870