Short communication: A33scFv-cytosine deaminase: a recombinant protein construct for antibody-directed enzyme-prodrug therapy

A recombinant fusion protein of colon carcinoma binding A33 single chain antibody with cytosine deaminase displayed specific antigen binding and enzyme activity in surface plasmon resonance and is catalytic activity assay. In vitro, it selectively increased the toxicity of 5-FC to A33 antigen-positi...

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Bibliographic Details
Published in:British journal of cancer 2003-03, Vol.88 (6), p.937-939
Main Authors: Deckert, P M, Renner, C, Cohen, L S, Jungbluth, A, Ritter, G, Bertino, J R, Old, L J, Welt, S
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Cancer research
Colon
Cytotoxicity
Enzymes
Experimental Therapeutics
Medical research
Proteins
RNA polymerase
Tumors
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Summary:A recombinant fusion protein of colon carcinoma binding A33 single chain antibody with cytosine deaminase displayed specific antigen binding and enzyme activity in surface plasmon resonance and is catalytic activity assay. In vitro, it selectively increased the toxicity of 5-FC to A33 antigen-positive cells by 300-fold, demonstrating the potency of this ADEPT strategy.
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6600751