Pathways Change in Expression During Replicative Aging in Saccharomyces cerevisiae

Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expressi...

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Bibliographic Details
Published in:The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2008-01, Vol.63 (1), p.21-34
Main Authors: Yiu, Gloria, McCord, Alejandra, Wise, Alison, Jindal, Rishi, Hardee, Jennifer, Kuo, Allen, Shimogawa, Michelle Yuen, Cahoon, Laty, Wu, Michelle, Kloke, John, Hardin, Johanna, Mays Hoopes, Laura L.
Format: Article
Language:English
Subjects:
Age
Aging
Aging - genetics
Biological variation
Changes
Daughters
Down-Regulation
Gene expression
Gene Expression Regulation, Fungal
Kinases
Molecular biology
Replicative aging
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
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Summary:Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes at 12 and 18–20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial environmental stress response. Our findings include a pseudostationary phase, downregulation of methylation-related metabolism, increased nucleotide excision repair–related mRNA, and a strong upregulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging.
ISSN:1079-5006
1758-535X
DOI:10.1093/gerona/63.1.21