Characterization of Structural Variations in the Peptidoglycan of Vancomycin-Susceptible Enterococcus faecium: Understanding Glycopeptide–Antibiotic Binding Sites Using Mass Spectrometry

• Published in: Journal of the American Society for Mass Spectrometry 2008-10, Vol.19 (10), p.1467-1475
• Main Authors: Patti, Gary J., Chen, Jiawei, Schaefer, Jacob, Gross, Michael L.
• Format: Article
• Language: English
• Subjects: Acetylation; Amides - analysis; Amines - analysis; Analytical Chemistry; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Binding Sites; Bioinformatics; Biological and medical sciences; Biotechnology; Cell Wall - physiology; Chemistry; Chemistry and Materials Science; Chromatography, Liquid - methods; Dipeptides - analysis; Enterococcus faecium; Enterococcus faecium - chemistry; Enterococcus faecium - growth & development; Enterococcus faecium - metabolism; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - metabolism; Hydroxylation; Magnetic Resonance Spectroscopy; Medical sciences; Models, Biological; Molecular Structure; Organic Chemistry; Peptides - analysis; Peptidoglycan - chemistry; Peptidoglycan - metabolism; Pharmacology. Drug treatments; Proteomics; Succinimides - analysis; Tandem Mass Spectrometry - methods; Vancomycin - chemistry; Vancomycin - metabolism
• ISSN: 1044-0305; 1879-1123
• DOI: 10.1016/j.jasms.2008.06.020

Enterococcus faecium, an opportunistic pathogen that causes a significant number of hospital-acquired infections each year, presents a serious clinical challenge because an increasing number of infections are resistant to the so-called antibiotic of last resort, vancomycin. Vancomycin and other new glycopeptide derivatives target the bacterial cell wall, thereby perturbing its biosynthesis. To help determine the modes of action of glycopeptide antibiotics, we have developed a bottom-up mass spectrometry approach complemented by solid-state nuclear magnetic resonance (NMR) to elucidate important structural characteristics of vancomycin-susceptible E. faecium peptidoglycan. Using accurate-mass measurements and integrating ion-current chromatographic peaks of digested peptidoglycan, we identified individual muropeptide species and approximated the relative amount of each. Even though the organism investigated is susceptible to vancomycin, only 3% of the digested peptidoglycan has the well-known d-Ala- d-Ala vancomycin-binding site. The data are consistent with a previously proposed template model of cell-wall biosynthesis where d-Ala- d-Ala stems that are not cross-linked are cleaved in mature peptidoglycan. Additionally, our mass-spectrometry approach allowed differentiation and quantification of muropeptide species seen as unresolved chromatographic peaks. Our method provides an estimate of the extent of muropeptides containing O-acetylation, amidation, hydroxylation, and the number of species forming cyclic imides. The varieties of muropeptides on which the modifications are detected suggest that significant processing occurs in mature peptidoglycan where several enzymes are active in editing cell-wall structure. LC/MS and solid-state NMR are presented as complementary techniques used to investigate the peptidoglycan of clinically relevant bacterial pathogens.