Mutations in components of the Wnt signaling pathway in gastric cancer

• Published in: World journal of gastroenterology : WJG 2008-03, Vol.14 (10), p.1570-1574
• Main Authors: Pan, Kai-Feng, Liu, Wan-Guo, Zhang, Lian, You, Wei-Cheng, Lu, You-Yong
• Format: Article
• Language: English
• Subjects: Axin Protein; AXIN1; AXIN2; beta Catenin - genetics; Case-Control Studies; Cytoskeletal Proteins - genetics; Exons - genetics; Female; Frameshift Mutation - genetics; Gene Expression Regulation, Neoplastic; Humans; Male; Mutation - genetics; Polymorphism, Single Nucleotide - genetics; Rapid Communication; Repressor Proteins - genetics; Signal Transduction - genetics; Stomach Neoplasms - genetics; Wnt Proteins - genetics; 聚合酶联反应; 致癌因子
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.14.1570

AIM： To explore the contribution of AXIN1, AXIN2 and beta-catenin, components of Wnt signaling pathway, to the carcinogenesis of gastric cancer （GC）, we examined AXIN1, AXIN2 exon7 and CTNNB1 （encoding beta- catenin） exon3 mutations in 70 GCs. METHODS： The presence of mutations was identified by polymerase chain reaction （PCR）-based denaturing high-performance liquid chromatography and direct DNA sequencing. Beta-catenin expression was detected by immunohistochemical analysis. RESULTS： Among the 70 GCs, 5 （7.1%） had mutations in one or two of these three components. A frameshift mutation （1 bp deletion） in exon7 of AXIN2 was found in one case. Four cases, including the case with a mutation in AXIN2, had frameshift mutations and missense mutations in AXIN1. Five single nucleotide polymorphisms （SNPs）, 334 C〉T, 874 C〉T, 1396 G〉A, 1690 C〉T and 1942 T〉G, were identified in AXIN1. A frameshift mutation （27 bp deletion） spanning exon3 of CTNNB1 was observed in one case. All four cases with mutations in AXIN1 and AXIN2 showed nuclear betacatenin expression. CONCLUSION： These data indicate that the mutationsin AXIN1 and AXIN2 may contribute to gastric carcinogenesis.