Mitochondrial activities in human cultured skin fibroblasts contaminated by Mycoplasma hyorhinis

Mycoplasma contaminations are a recurrent problem in the use of cultured cells, including human cells, especially as it has been shown to impede cell cycle, triggering cell death under various conditions. More specific consequences on cell metabolism are poorly known. Here we report the lack of sign...

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Bibliographic Details
Published in:BMC biochemistry 2003-11, Vol.4 (1), p.15-15, Article 15
Main Authors: Darin, Niklas, Kadhom, Norman, Brière, Jean-Jacques, Chretien, Dominique, Bébéar, Cécile M, Rötig, Agnès, Munnich, Arnold, Rustin, Pierre
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biochemistry, Molecular Biology
Cell Culture Techniques
Cell Respiration
Cells, Cultured
Cellular Biology
Child
Child, Preschool
Fibroblasts
Fibroblasts - enzymology
Fibroblasts - microbiology
Humans
Life Sciences
Microbiology and Parasitology
Middle Aged
Mitochondria
Mitochondria - enzymology
Mycoplasma
Mycoplasma hyorhinis
Mycoplasma hyorhinis - enzymology
Mycoplasma hyorhinis - isolation & purification
Phosphate Acetyltransferase
Phosphate Acetyltransferase - metabolism
Research Support, Non-U.S. Gov't
Skin
Skin - cytology
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Summary:Mycoplasma contaminations are a recurrent problem in the use of cultured cells, including human cells, especially as it has been shown to impede cell cycle, triggering cell death under various conditions. More specific consequences on cell metabolism are poorly known. Here we report the lack of significant consequence of a heavy contamination by the frequently encountered mycoplasma strain, M. hyorhinis, on the determination of respiratory chain activities, but the potential interference when assaying citrate synthase. Contamination by M. hyorhinis was detected by fluorescent imaging and further quantified by the determination of the mycoplasma-specific phosphate acetyltransferase activity. Noticeably, this latter activity was not found equally distributed in various mycoplasma types, being exceptionally high in M. hyorhinis. While we observed a trend for respiration reduction in heavily contaminated cells, no significant and specific targeting of any respiratory chain components could be identified. This suggested a potential interference with cell metabolism rather than direct interaction with respiratory chain components.
ISSN:1471-2091
1471-2091
DOI:10.1186/1471-2091-4-15