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Molecular Typing of Vibrio cholerae O1 Isolates from Thailand by Pulsed-field Gel Electrophoresis
The aim of the present study was to genotypically characterize Vibrio cholerae strains isolated from cholera patients in various provinces of Thailand. Two hundred and forty V. cholerae O1 strains, isolated from patients with cholera during two outbreaks, i.e. March 1999-April 2000 and December 2001...
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Published in: | Journal of health, population and nutrition population and nutrition, 2008-03, Vol.26 (1), p.79-87 |
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Main Authors: | , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | The aim of the present study was to genotypically characterize Vibrio
cholerae strains isolated from cholera patients in various provinces
of Thailand. Two hundred and forty V. cholerae O1 strains, isolated
from patients with cholera during two outbreaks, i.e. March 1999-April
2000 and December 2001-February 2002, in Thailand, were genotypically
characterized by NotI digestion and pulsed-field gel electrophoresis
(PFGE). In total, 17 PFGE banding patterns were found and grouped into
four Dice-coefficient clusters (PF-I to PF-IV). The patterns of V.
cholerae O1, El Tor reference strains from Australia, Peru, Romania,
and the United States were different from the patterns of reference
isolates from Asian countries, such as Bangladesh, India, and Thailand,
indicating a close genetic relationship or clonal origin of the
isolates in the same geographical region. The Asian reference strains,
regardless of their biotypes and serogroups (classical O1, El Tor O1,
O139, or O151), showed a genetic resemblance, but had different
patterns from the strains collected during the two outbreaks in
Thailand. Of 200 Ogawa strains collected during the first outbreak in
Thailand, two patterns (clones)-PF-I and PF-II-predominated, while
other isolates caused sporadic cases and were grouped together as
pattern PF-III. PF-II also predominated during the second outbreak, but
none of the 40 isolates (39 Inaba and 1 Ogawa) of the second outbreak
had the pattern PF-I; a minority showed a new pattern-PF-IV, and others
caused single cases, but were not groupable. In summary, this study
documented the sustained appearance of the pathogenic V. cholerae O1
clone PF-II, the disappearance of clones PF-I and PF-III, and the
emergence of new pathogenic clones during the two outbreaks of cholera.
Data of the study on molecular characteristics of indigenous V.
cholerae clinical isolates have public-health implications, not only
for epidemic tracing of existing strains but also for the recognition
of strains with new genotypes that may emerge in the future. |
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ISSN: | 1606-0997 2072-1315 |