DNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetes

Our group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in a mice model for tuberculosis. This protection was attributed to induction of a strong cellular immunity against HSP65. As specific i...

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Bibliographic Details
Published in:Journal of immune based therapies and vaccines 2009-09, Vol.7 (1), p.4-4, Article 4
Main Authors: Santos, Jr, Rubens R, Sartori, Alexandrina, Lima, Deison S, Souza, Patrícia Rm, Coelho-Castelo, Arlete Am, Bonato, Vânia Ld, Silva, Célio L
Format: Article
Language:English
Subjects:
Analysis
Complications and side effects
Diabetes
DNA vaccines
Genetic aspects
Health aspects
Mycobacterium
Original Research
Pancreatic diseases
Prevention
Risk factors
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Summary:Our group previously demonstrated that a DNA plasmid encoding the mycobacterial 65-kDa heat shock protein (DNA-HSP65) displayed prophylactic and therapeutic effect in a mice model for tuberculosis. This protection was attributed to induction of a strong cellular immunity against HSP65. As specific immunity to HSP60 family has been detected in arthritis, multiple sclerosis and diabetes, the vaccination procedure with DNA-HSP65 could induce a cross-reactive immune response that could trigger or worsen these autoimmune diseases. In this investigation was evaluated the effect of a previous vaccination with DNA-HSP65 on diabetes development induced by Streptozotocin (STZ). C57BL/6 mice received three vaccine doses or the corresponding empty vector and were then injected with multiple low doses of STZ. DNA-HSP65 vaccination protected mice from STZ induced insulitis and this was associated with higher production of IL-10 in spleen and also in the islets. This protective effect was also concomitant with the appearance of a regulatory cell population in the spleen and a decreased infiltration of the islets by T CD8+ lymphocytes. The vector (DNAv) also determined immunomodulation but its protective effect against insulitis was very discrete. The data presented in this study encourages a further investigation in the regulatory potential of the DNA-HSP65 construct. Our findings have important implications for the development of new immune therapy strategies to combat autoimmune diseases.
ISSN:1476-8518
1476-8518
DOI:10.1186/1476-8518-7-4