Quantification of nicotine, cotinine, trans-3′-hydroxycotinine, nornicotine and norcotinine in human meconium by liquid chromatography/tandem mass spectrometry

There are no analytical methods that simultaneously quantify nicotine, cotinine, trans-3′-hydroxycotinine, nornicotine and norcotinine in human meconium. Such a method could improve identification of in utero tobacco exposure, determine if maternal dose–meconium concentration relationships exist, an...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-02, Vol.863 (1), p.107-114
Main Authors: Gray, Teresa R., Shakleya, Diaa M., Huestis, Marilyn A.
Format: Article
Language:English
Subjects:
Adult
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid
Cotinine
Cotinine - analogs & derivatives
Cotinine - analysis
Female
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Indicators and Reagents
Infant, Newborn
Mass Spectrometry
Meconium
Meconium - chemistry
Medical sciences
Nicotine
Nicotine - analogs & derivatives
Nicotine - analysis
Pharmacology. Drug treatments
Pregnancy
Quality Control
Reference Standards
Smoking - metabolism
Tandem Mass Spectrometry
Tobacco
trans-3-Hydroxycotinine
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Summary:There are no analytical methods that simultaneously quantify nicotine, cotinine, trans-3′-hydroxycotinine, nornicotine and norcotinine in human meconium. Such a method could improve identification of in utero tobacco exposure, determine if maternal dose–meconium concentration relationships exist, and whether nicotine meconium concentrations predict neonatal outcomes. The first liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry method for simultaneous quantification of these analytes in meconium was developed and validated. Specimen preparation included homogenization, enzyme hydrolysis and solid phase extraction. The linear range was 1.25 or 5–500 ng/g. Method applicability was evaluated with meconium collected from an in utero tobacco exposed infant.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2008.01.001