Requirement of the activity of hepatocyte growth factor activator inhibitor type 1 for the extracellular appearance of a transmembrane serine protease matriptase in monkey kidney COS-1 cells

Hepatocyte growth factor activator inhibitor type I (HAI-1) is a membrane-bound, serine protease inhibitor with two protease-inhibitory domains (Kunitz domain I and II). HAI-1 is known as a physiological inhibitor of a membrane-bound serine protease, matriptase. Paradoxically, however, HAI-1 has bee...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2009-07, Vol.60 (1-3), p.95-103
Main Authors: Miyake, Yuka, Tsuzuki, Satoshi, Yasumoto, Makoto, Fushiki, Tohru, Inouye, Kuniyo
Format: Article
Language:English
Subjects:
Amino acids
Antibodies
Biochemistry
Biological and medical sciences
Biomedicine
Biotechnology
Chemistry
Chemistry and Materials Science
Enzymes
Fundamental and applied biological sciences. Psychology
Growth factors
Hepatocyte growth factor
Kidneys
Original Research
Peptides
Plasmids
Proteinase inhibitors
Proteins
Serine proteinase
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Summary:Hepatocyte growth factor activator inhibitor type I (HAI-1) is a membrane-bound, serine protease inhibitor with two protease-inhibitory domains (Kunitz domain I and II). HAI-1 is known as a physiological inhibitor of a membrane-bound serine protease, matriptase. Paradoxically, however, HAI-1 has been found to be required for the extracellular appearance of the protease in an expression system using a monkey kidney COS-1 cell line. In the present study, we show using COS-1 cells that co-expression of recombinant variants of HAI-1 with the inhibition activity toward matriptase, including a variant consisting only of Kunitz domain I (the domain responsible for inhibition of matriptase), allowed for the appearance of this protease in the conditioned medium, whereas that of the variants without the activity did not. These findings suggest that the inhibition activity toward matriptase is critical for the extracellular appearance of protease in COS-1 cells.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-009-9219-7