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Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes
Purpose By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocys...
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| Published in: | Journal of assisted reproduction and genetics 2009-12, Vol.26 (11-12), p.583-589 |
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| Main Authors: | , , , , , , , , |
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| container_end_page | 589 |
| container_issue | 11-12 |
| container_start_page | 583 |
| container_title | Journal of assisted reproduction and genetics |
| container_volume | 26 |
| creator | Liao, Hongqing Zhang, Shuoping Cheng, Dehua OuYang, Qi Lin, Ge Gu, Yifan Lu, Changfu Gong, Fei Lu, Guangxiu |
| description | Purpose
By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture.
Methods
After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding analysis was adopted to analyze the karyotype of 1PN hESCs.
Results
The diploid rate of blastocysts was 74.6%, which was significantly (
P
|
| doi_str_mv | 10.1007/s10815-009-9355-1 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2799555</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>21278538</sourcerecordid><originalsourceid>FETCH-LOGICAL-c565t-8a0a1b751dfcb74f5546e8706d070727a6d960b70ae5a2d7fc81a5fefb9517d33</originalsourceid><addsrcrecordid>eNp9kUtr3TAQhUVpyfsHZBNEF8nKrSRbr00hXPoIBLJJ10K2RjcOtnQr2QH310eXe0naQrPSwHznaGYOQueUfKKEyM-ZEkV5RYiudM15Rd-hI8plXcm6Ju9LTbiqSCPUITrO-ZEUULH6AB1SrXRTxEeoXS1TXEOAqe-wDXZYcp9x9PhhHm3AMLZpibl03L4OhcsTjLiDYcjYQeqfwGGf4ojHGOImxTB3A9iEfy_rOEE-RR-8HTKc7d8T9PPb1_vVj-r27vvN6vq26rjgU6UssbSVnDrftbLxnDcClCTCEUkkk1Y4LUgriQVumZO-U9RyD77VnEpX1yfoy853M7cjuA7ClOxgNqkfbVpMtL35uxP6B7OOT4ZJrTnnxeBqb5DirxnyZMY-b9e0AeKcTbmqEow1TSEv3yQZZVLxWhXw4z_gY5xTOfOWEYI2QugC0R3UpZhzAv8yMyVmG7TZBW1KfmYbtKFFc_Hnsq-KfbIFYDsgl1ZYQ3r9-f-uz_egtfg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>216614669</pqid></control><display><type>article</type><title>Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes</title><source>Springer Nature</source><source>PubMed Central</source><creator>Liao, Hongqing ; Zhang, Shuoping ; Cheng, Dehua ; OuYang, Qi ; Lin, Ge ; Gu, Yifan ; Lu, Changfu ; Gong, Fei ; Lu, Guangxiu</creator><creatorcontrib>Liao, Hongqing ; Zhang, Shuoping ; Cheng, Dehua ; OuYang, Qi ; Lin, Ge ; Gu, Yifan ; Lu, Changfu ; Gong, Fei ; Lu, Guangxiu</creatorcontrib><description>Purpose
By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture.
Methods
After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding analysis was adopted to analyze the karyotype of 1PN hESCs.
Results
The diploid rate of blastocysts was 74.6%, which was significantly (
P
< 0.001) higher than that of arrested cleavage-stage embryos (31.6%), and the diploid rate of hESCs was 97.0%, which was significantly (
P
< 0.01) higher than that of blastocysts; the haploid embryos were excluded by blastocyst culture; nevertheless, there still existed such chromosomal abnormalities as mosaic and monosomic in blastocysts and trisomy in hESCs.
Conclusions
Blastocyst culture is an effective method to select against chromosomal abnormalities, especially the haploids in 1PN embryos; however, development to the blastocyst stage is not a reliable marker for mosaicism or aneuploidy.</description><identifier>ISSN: 1058-0468</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1007/s10815-009-9355-1</identifier><identifier>PMID: 19894108</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Blastocyst - cytology ; Blastocyst - ultrastructure ; Chi-Square Distribution ; Chromosome Aberrations - embryology ; Chromosomes, Human ; Cytogenetic Analysis - methods ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - ultrastructure ; Ethanol ; Female ; Fetuses ; Genetics ; Gynecology ; Human Genetics ; Humans ; In Situ Hybridization, Fluorescence - methods ; Male ; Medicine ; Medicine & Public Health ; Ploidies ; Pregnancy ; Preimplantation Diagnosis - methods ; Reproductive Medicine ; Sex chromosomes ; Sperm ; Stem cells</subject><ispartof>Journal of assisted reproduction and genetics, 2009-12, Vol.26 (11-12), p.583-589</ispartof><rights>Springer Science+Business Media, LLC 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-8a0a1b751dfcb74f5546e8706d070727a6d960b70ae5a2d7fc81a5fefb9517d33</citedby><cites>FETCH-LOGICAL-c565t-8a0a1b751dfcb74f5546e8706d070727a6d960b70ae5a2d7fc81a5fefb9517d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799555/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799555/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19894108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liao, Hongqing</creatorcontrib><creatorcontrib>Zhang, Shuoping</creatorcontrib><creatorcontrib>Cheng, Dehua</creatorcontrib><creatorcontrib>OuYang, Qi</creatorcontrib><creatorcontrib>Lin, Ge</creatorcontrib><creatorcontrib>Gu, Yifan</creatorcontrib><creatorcontrib>Lu, Changfu</creatorcontrib><creatorcontrib>Gong, Fei</creatorcontrib><creatorcontrib>Lu, Guangxiu</creatorcontrib><title>Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><addtitle>J Assist Reprod Genet</addtitle><description>Purpose
By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture.
Methods
After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding analysis was adopted to analyze the karyotype of 1PN hESCs.
Results
The diploid rate of blastocysts was 74.6%, which was significantly (
P
< 0.001) higher than that of arrested cleavage-stage embryos (31.6%), and the diploid rate of hESCs was 97.0%, which was significantly (
P
< 0.01) higher than that of blastocysts; the haploid embryos were excluded by blastocyst culture; nevertheless, there still existed such chromosomal abnormalities as mosaic and monosomic in blastocysts and trisomy in hESCs.
Conclusions
Blastocyst culture is an effective method to select against chromosomal abnormalities, especially the haploids in 1PN embryos; however, development to the blastocyst stage is not a reliable marker for mosaicism or aneuploidy.</description><subject>Blastocyst - cytology</subject><subject>Blastocyst - ultrastructure</subject><subject>Chi-Square Distribution</subject><subject>Chromosome Aberrations - embryology</subject><subject>Chromosomes, Human</subject><subject>Cytogenetic Analysis - methods</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - ultrastructure</subject><subject>Ethanol</subject><subject>Female</subject><subject>Fetuses</subject><subject>Genetics</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Ploidies</subject><subject>Pregnancy</subject><subject>Preimplantation Diagnosis - methods</subject><subject>Reproductive Medicine</subject><subject>Sex chromosomes</subject><subject>Sperm</subject><subject>Stem cells</subject><issn>1058-0468</issn><issn>1573-7330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kUtr3TAQhUVpyfsHZBNEF8nKrSRbr00hXPoIBLJJ10K2RjcOtnQr2QH310eXe0naQrPSwHznaGYOQueUfKKEyM-ZEkV5RYiudM15Rd-hI8plXcm6Ju9LTbiqSCPUITrO-ZEUULH6AB1SrXRTxEeoXS1TXEOAqe-wDXZYcp9x9PhhHm3AMLZpibl03L4OhcsTjLiDYcjYQeqfwGGf4ojHGOImxTB3A9iEfy_rOEE-RR-8HTKc7d8T9PPb1_vVj-r27vvN6vq26rjgU6UssbSVnDrftbLxnDcClCTCEUkkk1Y4LUgriQVumZO-U9RyD77VnEpX1yfoy853M7cjuA7ClOxgNqkfbVpMtL35uxP6B7OOT4ZJrTnnxeBqb5DirxnyZMY-b9e0AeKcTbmqEow1TSEv3yQZZVLxWhXw4z_gY5xTOfOWEYI2QugC0R3UpZhzAv8yMyVmG7TZBW1KfmYbtKFFc_Hnsq-KfbIFYDsgl1ZYQ3r9-f-uz_egtfg</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Liao, Hongqing</creator><creator>Zhang, Shuoping</creator><creator>Cheng, Dehua</creator><creator>OuYang, Qi</creator><creator>Lin, Ge</creator><creator>Gu, Yifan</creator><creator>Lu, Changfu</creator><creator>Gong, Fei</creator><creator>Lu, Guangxiu</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20091201</creationdate><title>Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes</title><author>Liao, Hongqing ; Zhang, Shuoping ; Cheng, Dehua ; OuYang, Qi ; Lin, Ge ; Gu, Yifan ; Lu, Changfu ; Gong, Fei ; Lu, Guangxiu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-8a0a1b751dfcb74f5546e8706d070727a6d960b70ae5a2d7fc81a5fefb9517d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Blastocyst - cytology</topic><topic>Blastocyst - ultrastructure</topic><topic>Chi-Square Distribution</topic><topic>Chromosome Aberrations - embryology</topic><topic>Chromosomes, Human</topic><topic>Cytogenetic Analysis - methods</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - ultrastructure</topic><topic>Ethanol</topic><topic>Female</topic><topic>Fetuses</topic><topic>Genetics</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Ploidies</topic><topic>Pregnancy</topic><topic>Preimplantation Diagnosis - methods</topic><topic>Reproductive Medicine</topic><topic>Sex chromosomes</topic><topic>Sperm</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liao, Hongqing</creatorcontrib><creatorcontrib>Zhang, Shuoping</creatorcontrib><creatorcontrib>Cheng, Dehua</creatorcontrib><creatorcontrib>OuYang, Qi</creatorcontrib><creatorcontrib>Lin, Ge</creatorcontrib><creatorcontrib>Gu, Yifan</creatorcontrib><creatorcontrib>Lu, Changfu</creatorcontrib><creatorcontrib>Gong, Fei</creatorcontrib><creatorcontrib>Lu, Guangxiu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liao, Hongqing</au><au>Zhang, Shuoping</au><au>Cheng, Dehua</au><au>OuYang, Qi</au><au>Lin, Ge</au><au>Gu, Yifan</au><au>Lu, Changfu</au><au>Gong, Fei</au><au>Lu, Guangxiu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><stitle>J Assist Reprod Genet</stitle><addtitle>J Assist Reprod Genet</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>26</volume><issue>11-12</issue><spage>583</spage><epage>589</epage><pages>583-589</pages><issn>1058-0468</issn><eissn>1573-7330</eissn><abstract>Purpose
By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture.
Methods
After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding analysis was adopted to analyze the karyotype of 1PN hESCs.
Results
The diploid rate of blastocysts was 74.6%, which was significantly (
P
< 0.001) higher than that of arrested cleavage-stage embryos (31.6%), and the diploid rate of hESCs was 97.0%, which was significantly (
P
< 0.01) higher than that of blastocysts; the haploid embryos were excluded by blastocyst culture; nevertheless, there still existed such chromosomal abnormalities as mosaic and monosomic in blastocysts and trisomy in hESCs.
Conclusions
Blastocyst culture is an effective method to select against chromosomal abnormalities, especially the haploids in 1PN embryos; however, development to the blastocyst stage is not a reliable marker for mosaicism or aneuploidy.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>19894108</pmid><doi>10.1007/s10815-009-9355-1</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1058-0468 |
| ispartof | Journal of assisted reproduction and genetics, 2009-12, Vol.26 (11-12), p.583-589 |
| issn | 1058-0468 1573-7330 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2799555 |
| source | Springer Nature; PubMed Central |
| subjects | Blastocyst - cytology Blastocyst - ultrastructure Chi-Square Distribution Chromosome Aberrations - embryology Chromosomes, Human Cytogenetic Analysis - methods Embryonic Stem Cells - cytology Embryonic Stem Cells - ultrastructure Ethanol Female Fetuses Genetics Gynecology Human Genetics Humans In Situ Hybridization, Fluorescence - methods Male Medicine Medicine & Public Health Ploidies Pregnancy Preimplantation Diagnosis - methods Reproductive Medicine Sex chromosomes Sperm Stem cells |
| title | Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes |
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