Commercial DNA Probes for Mycobacteria Incorrectly Identify a Number of Less Frequently Encountered Species

Although commercially available DNA probes for identification of mycobacteria have been investigated with large numbers of strains, nothing is known about the ability of these probes to identify less frequently encountered species. We analyzed, with INNO LiPA MYCOBACTERIA (Innogenetics) and with Gen...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2010-01, Vol.48 (1), p.307-310
Main Authors: Tortoli, Enrico, Pecorari, Monica, Fabio, Giuliana, Messinò, Massimino, Fabio, Anna
Format: Article
Language:English
Subjects:
Bacteriological Techniques - methods
Bacteriology
Biological and medical sciences
Cross Reactions
Diagnostic Errors
DNA Probes - genetics
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Miscellaneous
Molecular Diagnostic Techniques - methods
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium
Mycobacterium - classification
Mycobacterium - genetics
Mycobacterium - isolation & purification
Mycobacterium avium
Mycobacterium fortuitum
Mycobacterium Infections - diagnosis
Mycobacterium Infections - microbiology
Reagent Kits, Diagnostic
Sensitivity and Specificity
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Summary:Although commercially available DNA probes for identification of mycobacteria have been investigated with large numbers of strains, nothing is known about the ability of these probes to identify less frequently encountered species. We analyzed, with INNO LiPA MYCOBACTERIA (Innogenetics) and with GenoType Mycobacterium (Hein), 317 strains, belonging to 136 species, 61 of which had never been assayed before. INNO LiPA misidentified 20 taxa, the majority of which cross-reacted with the probes specific for Mycobacterium fortuitum and the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum group. GenoType misidentified 28 taxa, most of which cross-reacted with M. intracellulare and M. fortuitum probes; furthermore, eight species were not recognized as members of the genus MYCOBACTERIUM: Among 54 strains investigated with AccuProbe (Gen-Probe), cross-reactions were detected for nine species, with the probes aiming at the M. avium complex being most involved in cross-reactions.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01536-09