Isolation and characterization of a novel 2-sec-butylphenol-degrading bacterium Pseudomonas sp. strain MS-1

• Published in: Biodegradation (Dordrecht) 2010-04, Vol.21 (2), p.157-165
• Main Authors: Toyama, Tadashi, Maeda, Noritaka, Murashita, Manabu, Chang, Yong-Cheol, Kikuchi, Shintaro
• Format: Article
• Language: English
• Subjects: Analysis; Aquatic Pollution; Bacteria; Biodegradation; Biodegradation of pollutants; Biodegradation, Environmental; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Energy sources; Environment and pollution; Fresh water; Fundamental and applied biological sciences. Psychology; Gas chromatography; Geochemistry; Geologic Sediments - microbiology; Industrial applications and implications. Economical aspects; Life Sciences; Mass spectrometry; Metabolites; Microbiology; Molecular Sequence Data; Original Paper; Phenols - chemistry; Phenols - metabolism; Phylogeny; Pseudomonas - classification; Pseudomonas - genetics; Pseudomonas - isolation & purification; Pseudomonas - metabolism; Sediments (Geology); Soil Science & Conservation; Terrestrial Pollution; Waste Management/Waste Technology; Waste Water Technology; Water Management; Water pollution; Water Pollution Control
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-009-9290-y

A novel bacterium capable of utilizing 2- sec -butylphenol as the sole carbon and energy source, Pseudomonas sp. strain MS-1, was isolated from freshwater sediment. Within 30 h, strain MS-1 completely degraded 1.5 mM 2- sec -butylphenol in basal salt medium, with concomitant cell growth. A pathway for the metabolism of 2- sec -butylphenol by strain MS-1 was proposed on the basis of the identification of 3 internal metabolites—3- sec -butylcatechol, 2-hydroxy-6-oxo-7-methylnona-2,4-dienoic acid, and 2-methylbutyric acid—by gas chromatography-mass spectrometry analysis. Strain MS-1 degraded 2- sec -butylphenol through 3- sec -butylcatechol along a meta -cleavage pathway. Degradation experiments with various alkylphenols showed that the degradability of alkylphenols by strain MS-1 depended strongly on the position ( ortho  ≫  meta  =  para ) of the alkyl substitute, and that strain MS-1 could degrade 2-alkylphenols with various sized and branched alkyl chain ( o -cresol, 2-ethylphenol, 2- n -propylphenol, 2-isopropylphenol, 2- sec -butylphenol, and 2- tert -butylphenol), as well as a dialkylphenol (namely, 6- tert -butyl- m -cresol).