Genetic Evolution of Bacteriophage, II. Physical Length of the Homologous Region in a Hybrid between Serologically and Morphologically Unrelated Phages

• Published in: Proceedings of the National Academy of Sciences - PNAS 1970-06, Vol.66 (2), p.385-389
• Main Authors: Akiyoshi, Hiroto, Yamamoto, Nobuto
• Format: Article
• Language: English
• Subjects: Bacteriophages; Biological Sciences: Genetics; Carbon Isotopes; Centrifugation; Centrifugation, Density Gradient; Citrates; DNA; DNA, Viral - isolation & purification; Evolutionary genetics; Genetics, Microbial; Genomes; Hybridity; Hybridization, Genetic; Medical genetics; Microbial genetics; Molecular weight; Salmonella Phages; Salmonella typhimurium; Thymidine; Thymine - pharmacology; Tritium
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.66.2.385

P22 and P221 DNA were labeled with 3H-thymidine and hybridized with P22 DNA, P221 DNA, or Fels 1 DNA immobilized on Millipore membrane filters. With this technique, 31% of the P22 genome was computed to be homologous to the P221 genome. Since the molecular weight of P22 DNA is 2.65 × 107 daltons, the homologous region between P22 and P221 should be equivalent to about 8.2 × 106 daltons and contain 13,300 nucleotide pairs or about 4400 triplets. Moreover, 46% of the P221 genome was computed to be homologous to the P22 genome. Therefore, the molecular weight of P221 DNA was calculated to be about 1.80 × 107 daltons. Sucrose gradient centrifugation showed that the molecular weight of P221 DNA was 2.01 × 107 daltons. Thus, the P221 genome is about 25 to 30% smaller than the P22 genome. Furthermore, 54% of the P221 genome was found to be homologous to the Fels 1 genome. However, the labeled P22 DNA hybridized with Fels 1 DNA was about 2% of the labeled P22 DNA hybridized with P22 DNA.