Crystallization and X-ray diffraction studies of inverting trehalose phosphorylase from Thermoanaerobacter sp

• Published in: Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2010-04, Vol.66 (4), p.442-447
• Main Authors: Van Hoorebeke, Annelies, Stout, Jan, Van der Meeren, Ruben, Kyndt, John, Van Beeumen, Jozef, Savvides, Savvas N.
• Format: Article
• Language: English
• Subjects: crystal dehydration; Crystallization; Crystallization Communications; Crystallography, X-Ray; disaccharide phosphorylases; Escherichia coli; GH65; Glucosyltransferases - chemistry; inverting; Models, Molecular; Protein Structure, Quaternary; Thermoanaerobacter; Thermoanaerobacter - enzymology; trehalose phosphorylase
• ISSN: 1744-3091; 1744-3091; 2053-230X
• DOI: 10.1107/S1744309110005749

Disaccharide phosphorylases are attractive enzymatic platforms for tailor‐made sugar synthesis owing to their ability to catalyze both the synthesis and the breakdown of disaccharides. Trehalose phosphorylase from Thermoanaerobacter sp. (TP) is a glycoside hydrolase family 65 enzyme which catalyzes the reversible breakdown of trehalose [d‐glucopyranosyl‐α(1,1)α‐d‐glucopyranose] to β‐d‐glucose 1‐phosphate and d‐glucose. Recombinant purified protein was produced in Escherichia coli and crystallized in space group P212121. Crystals of recombinant TP were obtained in their native form and were soaked with glucose, with n‐octyl‐β‐d‐glucoside and with trehalose. The crystals presented a number of challenges including an unusually large unit cell, with a c axis measuring 420 Å, and variable diffraction quality. Crystal‐dehydration protocols led to improvements in diffraction quality that were often dramatic, typically from 7–8 to 3–4 Å resolution. The structure of recombinant TP was determined by molecular replacement to 2.8 Å resolution, thus establishing a starting point for investigating the structural and mechanistic determinants of the disaccharide phosphorylase activity. To the best of our knowledge, this is the first crystal structure determination of an inverting trehalose phosphorylase.