Influence of the extracellular matrix and integrins on volume-sensitive osmolyte anion channels in C2C12 myoblasts

The purpose of this study was to determine whether extracellular matrix (ECM) composition through integrin receptors modulated the volume-sensitive osmolyte anion channels (VSOACs) in skeletal muscle-derived C2C12 cells. Cl(-) currents were recorded in whole cell voltage-clamped cells grown on lamin...

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Bibliographic Details
Published in:American Journal of Physiology: Cell Physiology 2010-05, Vol.298 (5), p.C1006-C1017
Main Authors: Neveux, Iva, Doe, Jinger, Leblanc, Normand, Valencik, Maria L
Format: Article
Language:English
Subjects:
Acidosis - blood
Acidosis - chemically induced
Acidosis - metabolism
Acidosis - urine
Animals
Caspase 3 - metabolism
Cell Death
Cells
Chloride Channels - metabolism
Extracellular Matrix - metabolism
Gene Expression Regulation
Glutamic Acid
Hippocampus - cytology
Hippocampus - metabolism
Humans
Hydrochloric Acid - pharmacology
Integrins - metabolism
Kinetics
Membrane Transporters, Ion Channels and Pumps
Membranes
Mice
Musculoskeletal system
Myoblasts - physiology
Neurons - metabolism
Physiology
Protein Transport
Proteins
Rats
Rats, Sprague-Dawley
Ribonucleic acid
RNA
Sodium-Bicarbonate Symporters - genetics
Sodium-Bicarbonate Symporters - metabolism
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Summary:The purpose of this study was to determine whether extracellular matrix (ECM) composition through integrin receptors modulated the volume-sensitive osmolyte anion channels (VSOACs) in skeletal muscle-derived C2C12 cells. Cl(-) currents were recorded in whole cell voltage-clamped cells grown on laminin (LM), fibronectin (FN), or in the absence of a defined ECM (NM). Basal membrane currents recorded in isotonic media (300 mosmol/kg) were larger in cells grown on FN (3.8-fold at +100 mV) or LM (8.8-fold at +100 mV) when compared with NM. VSOAC currents activated by cell exposure to hypotonic solution were larger in cells grown on LM (1.72-fold at +100 mV) or FN (1.75-fold at +100 mV) compared with NM. Additionally, the kinetics of VSOAC activation was approximately 27% quicker on FN and LM. These currents were tamoxifen sensitive, displayed outward rectification, reversed at the equilibrium potential of Cl(-) and inactivated at potentials >+60 mV. Specific knockdown of beta(1)-integrin by short hairpin RNA interference strongly inhibited the VSOAC Cl(-) currents in cells plated on FN. In conclusion, ECM composition and integrins profoundly influence the biophysical properties and mechanisms of onset of VSOACs.
ISSN:0363-6143
1522-1563
DOI:10.1152/ajpcell.00359.2009