12/15-Lipoxygenase Products Induce Inflammation and Impair Insulin Signaling in 3T3-L1 Adipocytes

• Published in: Obesity (Silver Spring, Md.) Md.), 2009-09, Vol.17 (9), p.1657-1663
• Main Authors: Chakrabarti, Swarup K., Cole, Banumathi K., Wen, Yeshao, Keller, Susanna R., Nadler, Jerry L.
• Format: Article
• Language: English
• Subjects: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - metabolism; 3T3-L1 Cells; Adipocytes; Adipocytes - enzymology; Adiponectin - metabolism; Animals; Arachidonate 12-Lipoxygenase - genetics; Arachidonate 12-Lipoxygenase - metabolism; Arachidonate 15-Lipoxygenase - genetics; Arachidonate 15-Lipoxygenase - metabolism; Atherosclerosis; Body fat; Calories; Cell Differentiation; Cytokines; Cytokines - metabolism; Diabetes; Diet; Disease Models, Animal; Gene Expression Regulation, Enzymologic; Inflammation; Inflammation - enzymology; Inflammation - physiopathology; Inflammation Mediators - metabolism; Insulin - metabolism; Insulin Receptor Substrate Proteins - metabolism; Insulin Resistance; Kinases; Leukocytes; Leukotrienes - metabolism; Male; Mice; Mice, Inbred C57BL; Mitogen-Activated Protein Kinase 8 - metabolism; Obesity; Obesity - enzymology; Obesity - physiopathology; Palmitic Acid - metabolism; Phosphorylation; Proteins; Proto-Oncogene Proteins c-akt - metabolism; RNA, Messenger - metabolism; Signal Transduction; Time Factors; Tumor necrosis factor-TNF; Up-Regulation
• ISSN: 1930-7381; 1930-739X
• DOI: 10.1038/oby.2009.192

Inflammation and insulin resistance associated with visceral obesity are important risk factors for the development of type 2 diabetes, atherosclerosis, and the metabolic syndrome. The 12/15‐lipoxygenase (12/15‐LO) enzyme has been linked to inflammatory changes in blood vessels that precede the development of atherosclerosis. The expression and role of 12/15‐LO in adipocytes have not been evaluated. We found that 12/15‐LO mRNA was dramatically upregulated in white epididymal adipocytes of high‐fat fed mice. 12/15‐LO was poorly expressed in 3T3‐L1 fibroblasts and was upregulated during differentiation into adipocytes. Interestingly, the saturated fatty acid palmitate, a major component of high fat diets, augmented expression of 12/15‐LO in vitro. When 3T3‐L1 adipocytes were treated with the 12/15‐LO products, 12‐hydroxyeicosatetranoic acid (12(S)‐HETE) and 12‐hydroperoxyeicosatetraenoic acid (12(S)‐HPETE), expression of proinflammatory cytokine genes, including tumor necrosis factor‐α (TNF‐α), monocyte chemoattractant protein 1 (MCP‐1), interleukin 6 (IL‐6), and IL‐12p40, was upregulated whereas anti‐inflammatory adiponectin gene expression was downregulated. 12/15‐LO products also augmented c‐Jun N‐terminal kinase 1 (JNK‐1) phosphorylation, a known negative regulator of insulin signaling. Consistent with impaired insulin signaling, we found that insulin‐stimulated 3T3‐L1 adipocytes exhibited decreased IRS‐1(Tyr) phosphorylation, increased IRS‐1(Ser) phosphorylation, and impaired Akt phosphorylation when treated with 12/15‐LO product. Taken together, our data suggest that 12/15‐LO products create a proinflammatory state and impair insulin signaling in 3T3‐L1 adipocytes. Because 12/15‐LO expression is upregulated in visceral adipocytes by high‐fat feeding in vivo and also by addition of palmitic acid in vitro, we propose that 12/15‐LO plays a role in promoting inflammation and insulin resistance associated with obesity.