Ultraviolet B Radiation of Human Skin Generates Platelet-activating Factor Receptor Agonists

Ultraviolet B radiation (UVB) is a potent stimulator of epidermal cytokine production. In addition to cytokines, such as tumor necrosis factor‐alpha (TNF‐α), UVB generates bioactive lipids including platelet‐activating factor (PAF). Our previous in vitro studies in keratinocytes or epithelial cell l...

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Bibliographic Details
Published in:Photochemistry and photobiology 2010-07, Vol.86 (4), p.949-954
Main Authors: Travers, Jared B., Berry, Damien, Yao, Yongxue, Yi, Qiaofang, Konger, Raymond L., Travers, Jeffrey B.
Format: Article
Language:English
Subjects:
Antioxidants - pharmacology
Apoptosis
Ascorbic Acid - pharmacology
Dose-Response Relationship, Radiation
Experiments
Free radicals
Humans
Kinases
Lipids
Mass spectrometry
Oxidative Stress
Phosphorylcholine - antagonists & inhibitors
Phosphorylcholine - metabolism
Phosphorylcholine - pharmacology
Platelet Membrane Glycoproteins - agonists
Proteins
Quinazolines - pharmacology
Reactive Oxygen Species - metabolism
Receptors, G-Protein-Coupled - agonists
Rodents
Skin - metabolism
Skin - radiation effects
Time Factors
Ultraviolet Rays
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Summary:Ultraviolet B radiation (UVB) is a potent stimulator of epidermal cytokine production. In addition to cytokines, such as tumor necrosis factor‐alpha (TNF‐α), UVB generates bioactive lipids including platelet‐activating factor (PAF). Our previous in vitro studies in keratinocytes or epithelial cell lines have demonstrated that UVB‐mediated production of PAF agonists is due primarily to the pro‐oxidative effects of this stimulant, resulting in the nonenzymatic production of modified phosphocholines (oxidized glycerophosphocholines). The current studies use human skin to assess whether UVB irradiation generates PAF‐receptor agonists, and the role of oxidative stress in their production. These studies demonstrate that UVB irradiation of human skin results in PAF agonists, which are blocked by the antioxidant vitamin C and the epidermal growth factor receptor inhibitor PD168393. Inasmuch as UVB‐generated PAF agonists have been implicated in animal model systems as being involved in photobiologic processes including systemic immunosuppression and cytokine (TNF‐α) production, these studies indicate that this novel activity could be involved in human disease.
ISSN:0031-8655
1751-1097
DOI:10.1111/j.1751-1097.2010.00743.x