Possibilities and pitfalls in quantifying the extent of cysteine sulfenic acid modification of specific proteins within complex biofluids

Cysteine sulfenic acid (Cys-SOH) plays important roles in the redox regulation of numerous proteins. As a relatively unstable posttranslational protein modification it is difficult to quantify the degree to which any particular protein is modified by Cys-SOH within a complex biological environment....

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Bibliographic Details
Published in:BMC biochemistry 2010-07, Vol.11 (1), p.25-25
Main Authors: Rehder, Douglas S, Borges, Chad R
Format: Article
Language:English
Subjects:
Antibodies
Cyclohexanones - chemistry
Cysteine
Cysteine - analogs & derivatives
Cysteine - chemistry
Cysteine - metabolism
Humans
Nitrobenzoates - chemistry
Oxidation-Reduction
Physiological aspects
Prealbumin - chemistry
Proteins
Serum Albumin - chemistry
Spectrometry, Mass, Electrospray Ionization
Sulfenic Acids - chemistry
Sulfenic Acids - metabolism
Sulfhydryl Compounds - chemistry
Thiols
Viral antibodies
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Summary:Cysteine sulfenic acid (Cys-SOH) plays important roles in the redox regulation of numerous proteins. As a relatively unstable posttranslational protein modification it is difficult to quantify the degree to which any particular protein is modified by Cys-SOH within a complex biological environment. The goal of these studies was to move a step beyond detection and into the relative quantification of Cys-SOH within specific proteins found in a complex biological setting--namely, human plasma. This report describes the possibilities and limitations of performing such analyses based on the use of thionitrobenzoic acid and dimedone-based probes which are commonly employed to trap Cys-SOH. Results obtained by electrospray ionization-based mass spectrometric immunoassay reveal the optimal type of probe for such analyses as well as the reproducible relative quantification of Cys-SOH within albumin and transthyretin extracted from human plasma--the latter as a protein previously unknown to be modified by Cys-SOH. The relative quantification of Cys-SOH within specific proteins in a complex biological setting can be accomplished, but several analytical precautions related to trapping, detecting, and quantifying Cys-SOH must be taken into account prior to pursuing its study in such matrices.
ISSN:1471-2091
1471-2091
DOI:10.1186/1471-2091-11-25