Short-term storage and transport at cold temperatures of 2-cell mouse embryos produced by cryopreserved sperm

At refrigerated temperatures, mouse embryos can maintain developmental ability for short periods. Previously, we succeeded in transporting vitrified and warmed 2-cell mouse embryos while maintaining developmental ability at refrigerated temperatures for 50 h. Transport of nonfrozen embryos is an eas...

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Bibliographic Details
Published in:Journal of the American Association for Laboratory Animal Science 2010-07, Vol.49 (4), p.415-419
Main Authors: Takeo, Toru, Kondo, Tomoko, Haruguchi, Yukie, Fukumoto, Kiyoko, Nakagawa, Yoshiko, Takeshita, Yumi, Nakamuta, Yuko, Tsuchiyama, Shuuji, Shimizu, Norihiko, Hasegawa, Takanori, Goto, Motohito, Miyachi, Hitoshi, Anzai, Masayuki, Fujikawa, Rie, Nomaru, Koji, Kaneko, Takehito
Format: Article
Language:English
Subjects:
animal transport
Animals
blastocyst
cold storage
Cold Temperature
Cryopreservation
Culture Media
embryo (animal)
Embryo Culture Techniques
Embryo Transfer
embryogenesis
Embryonic Development
Female
Fertilization in Vitro
in vitro fertilization
laboratory animals
Male
methodology
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
refrigeration
Reproduction
Spermatozoa
storage temperature
temperature profiles
transgenic animals
Transportation
viability
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Summary:At refrigerated temperatures, mouse embryos can maintain developmental ability for short periods. Previously, we succeeded in transporting vitrified and warmed 2-cell mouse embryos while maintaining developmental ability at refrigerated temperatures for 50 h. Transport of nonfrozen embryos is an easier and more useful means of exchanging genetically engineered mice between laboratories than is transport of cryopreserved embryos. Here we examined the developmental ability of transported 2-cell embryos that were produced through in vitro fertilization using cryopreserved sperm. Results show that 2-cell embryos produced by cryopreserved sperm can develop into blastocysts after cold storage for 24, 48, and 72 h. Transported 2-cell embryos produced by cryopreserved sperm yielded a favorable number of pups in all of the receiving laboratories after transport lasting 48 to 52 h. In summary, cold storage and transport of 2-cell embryos derived from cryopreserved sperm at refrigerated temperatures provides a novel means of transporting genetically engineered mice as an alternative to the transport of cryopreserved embryos and sperm.
ISSN:1559-6109
2769-6677