Laser Desorption Postionization Mass Spectrometry of Antibiotic-Treated Bacterial Biofilms Using Tunable Vacuum Ultraviolet Radiation

Laser desorption postionization mass spectrometry (LDPI-MS) with 8.0−12.5 eV vacuum ultraviolet synchrotron radiation is used to single photon ionize antibiotics and extracellular neutrals that are laser desorbed both from neat and intact bacterial biofilms. Neat antibiotics are optimally detected u...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2010-09, Vol.82 (17), p.7472-7478
Main Authors: Gasper, Gerald L, Takahashi, Lynelle K, Zhou, Jia, Ahmed, Musahid, Moore, Jerry F, Hanley, Luke
Format: Article
Language:English
Subjects:
Analytical chemistry
Anti-Bacterial Agents - pharmacology
ANTIBIOTICS
BACTERIA
Biofilms
Biofilms - drug effects
Chemistry
DESORPTION
Exact sciences and technology
FAR ULTRAVIOLET RADIATION
GENERAL AND MISCELLANEOUS
INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
IONIZATION
Ions
LASER RADIATION
Laser, Desorption, Postionization, Spectrometry, Antibiotic-Treated, Bacterial Biofilms, Tunable Vacuum Ultraviolet Radiation
Mass spectrometry
MASS SPECTROSCOPY
Rifampin - pharmacology
SENSITIVITY
Spectrometric and optical methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Staphylococcus epidermidis - physiology
SYNCHROTRON RADIATION
Ultraviolet radiation
Ultraviolet Rays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Laser desorption postionization mass spectrometry (LDPI-MS) with 8.0−12.5 eV vacuum ultraviolet synchrotron radiation is used to single photon ionize antibiotics and extracellular neutrals that are laser desorbed both from neat and intact bacterial biofilms. Neat antibiotics are optimally detected using 10.5 eV LDPI-MS but can be ionized using 8.0 eV radiation, in agreement with prior work using 7.87 eV LDPI-MS. Tunable vacuum ultraviolet radiation also postionizes laser desorbed neutrals of antibiotics and extracellular material from within intact bacterial biofilms. Different extracellular material is observed by LDPI-MS in response to rifampicin or trimethoprim antibiotic treatment. Once again, 10.5 eV LDPI-MS displays the optimum trade-off between improved sensitivity and minimum fragmentation. Higher energy photons at 12.5 eV produce significant parent ion signal, but fragment intensity and other low mass ions are also enhanced. No matrix is added to enhance desorption, which is performed at peak power densities insufficient to directly produce ions, thus allowing observation of true VUV postionization mass spectra of antibiotic treated biofilms.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac101667q