Enhanced Bone Morphogenetic Protein-2-Induced Ectopic and Orthotopic Bone Formation by Intermittent Parathyroid Hormone (1–34) Administration

Bone morphogenetic proteins (BMPs) play a central role in local bone regeneration strategies, whereas the anabolic features of parathyroid hormone (PTH) are particularly appealing for the systemic treatment of generalized bone loss. The aim of the current study was to investigate whether local BMP-2...

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Bibliographic Details
Published in:Tissue engineering. Part A 2010-12, Vol.16 (12), p.3769-3777
Main Authors: Kempen, Diederik H.R., Lu, Lichun, Hefferan, Theresa E., Creemers, Laura B., Heijink, Andras, Maran, Avudaiappan, Dhert, Wouter J.A., Yaszemski, Michael J.
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - chemistry
Bone Density - drug effects
Bone Morphogenetic Protein 2 - pharmacology
Bone Regeneration - drug effects
Bones
Cell growth
Femur - surgery
Fumarates - chemistry
Gelatin - chemistry
Hormones
Lactic Acid - chemistry
Original
Original Articles
Osteogenesis - drug effects
Parathyroid Hormone - administration & dosage
Parathyroid Hormone - pharmacology
Polyglycolic Acid - chemistry
Polypropylenes - chemistry
Rats
Tissue engineering
Tissue Engineering - methods
X-Ray Microtomography
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Summary:Bone morphogenetic proteins (BMPs) play a central role in local bone regeneration strategies, whereas the anabolic features of parathyroid hormone (PTH) are particularly appealing for the systemic treatment of generalized bone loss. The aim of the current study was to investigate whether local BMP-2-induced bone regeneration could be enhanced by systemic administration of PTH (1–34). Empty or BMP-2-loaded poly(lactic-co glycolic acid)/poly(propylene fumarate)/gelatin composites were implanted subcutaneously and in femoral defects in rats ( n  = 9). For the orthotopic site, empty defects were also tested. Each of the conditions was investigated in combination with daily administered subcutaneous PTH (1–34) injections in the neck. After 8 weeks of implantation, bone mineral density (BMD) and bone volume were analyzed using microcomputed tomography and histology. Ectopic bone formation and almost complete healing of the femoral defect were only seen in rats that received BMP-2-loaded composites. Additional treatment of the rats with PTH (1–34) resulted in significantly ( p  
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2010.0173