Involvement of a Nucleolar Component, Perichromonucleolin, in the Condensation and Decondensation of Chromosomes

A component of the nucleolar material, perichromonucleolin (PCN), was identified by a specific antiserum against nucleoli obtained from a scleroderma patient. The distribution and changes of PCN during the mitotic cycle were followed using this antiserum and indirect immunofluorescence microscopy. B...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-11, Vol.84 (22), p.7953-7956
Main Authors: Luji, Shi, Zumei, Ni, Shi, Zhao, Ge, Wang, Yang Yang
Format: Article
Language:English
Subjects:
Anaphase
Animals
Antiserum
Ascites
Autoantibodies - immunology
Biological and medical sciences
Cell nucleolus
Cell Nucleolus - analysis
Cell Nucleolus - physiology
Chromatin. Chromosome
Chromosomes
Chromosomes - ultrastructure
Cross Reactions
Fundamental and applied biological sciences. Psychology
Humans
Interphase
Liver - analysis
Metaphase
Mice
Mitosis
Molecular and cellular biology
Molecular genetics
Prophase
Scleroderma, Systemic - immunology
Telophase
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Summary:A component of the nucleolar material, perichromonucleolin (PCN), was identified by a specific antiserum against nucleoli obtained from a scleroderma patient. The distribution and changes of PCN during the mitotic cycle were followed using this antiserum and indirect immunofluorescence microscopy. Based upon the behavior of PCN during mitosis, it could be differentiated into chromosomal and nonchromosomal portions. During prophase the former gradually associates with the surface of the condensing chromosomes and forms the coat or pellicle around the metaphase and anaphase chromosomes. This pellicle PCN is carried by the anaphase chromosomes to the daughter nuclei. During the time from telophase to interphase, the pellicle PCN dissociates from the decondensing chromosomes and is incorporated into the new nucleoli. The nonchromosomal PCN, after the breakdown of the nuclear membrane, distributes in the cytoplasmic region around the condensed chromosomes and diffuses into the cytoplasm during anaphase. Preliminary biochemical analysis by immunoblotting showed that the PCN consists of two main proteins with molecular masses of 36 kDa and 30 kDa.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.22.7953