KAT1 is Not Essential for Stomatal Opening

It is generally accepted that K+ uptake into guard cells via inward-rectifying K+ channels is required for stomatal opening. To test whether the guard cell K+ channel KAT1 is essential for stomatal opening, a knockout mutant, KAT1::En-1, was isolated from an En-1 mutagenized Arabidopsis thaliana pop...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-02, Vol.98 (5), p.2917-2921
Main Authors: Szyroki, Alexander, Ivashikina, Natalya, Dietrich, Petra, M. Rob G. Roelfsema, Ache, Peter, Reintanz, Birgit, Deeken, Rosalia, Godde, Matthias, Felle, Hubert, Steinmeyer, Ralf, Palme, Klaus, Hedrich, Rainer
Format: Article
Language:English
Subjects:
Arabidopsis - physiology
Arabidopsis Proteins
Base Sequence
Biological Sciences
Botany
Cells
DNA Primers
DNA Transposable Elements
Electric potential
Electrodes
Flowers & plants
Guard cells
Leaves
Messenger RNA
Mutation
Patch-Clamp Techniques
Plant Proteins
Plants
Potassium
Potassium Channels - genetics
Potassium Channels - physiology
Potassium Channels, Inwardly Rectifying
Protoplasts
Reverse transcriptase polymerase chain reaction
Stomata
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Summary:It is generally accepted that K+ uptake into guard cells via inward-rectifying K+ channels is required for stomatal opening. To test whether the guard cell K+ channel KAT1 is essential for stomatal opening, a knockout mutant, KAT1::En-1, was isolated from an En-1 mutagenized Arabidopsis thaliana population. Stomatal action and K+ uptake, however, were not impaired in KAT1-deficient plants. Reverse transcription-PCR experiments with isolated guard cell protoplasts showed that in addition to KAT1, the K+ channels AKT1, AKT2/3, AtKC1, and KAT2 were expressed in this cell type. In impalement measurements, intact guard cells exhibited inward-rectifying K+ currents across the plasma membrane of both wild-type and KAT1::En-1 plants. This study demonstrates that multiple K+ channel transcripts exist in guard cells and that KAT1 is not essential for stomatal action.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.051616698