Macrophages recognize streptococci through bacterial single-stranded RNA

Group B streptococcus (GBS) is a leading cause of both neonatal sepsis and meningitis, two diseases that are characterized by inflammation. However, the manner in which GBS organisms are recognized by monocytes and macrophages is poorly understood. In this study, we report that the recognition of GB...

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Bibliographic Details
Published in:EMBO reports 2011-01, Vol.12 (1), p.71-76
Main Authors: Deshmukh, Sachin D, Kremer, Bernhard, Freudenberg, Marina, Bauer, Stefan, Golenbock, Douglas T, Henneke, Philipp
Format: Article
Language:English
Subjects:
adaptor proteins
Animals
Bacteria
bacterial infection
Base Sequence
Cytokines - biosynthesis
EMBO19
EMBO23
EMBO36
Gram-positive bacteria
Host-Pathogen Interactions
Humans
Infant, Newborn
Inflammation
Macrophages
Macrophages - immunology
Macrophages - microbiology
Membrane Transport Proteins - immunology
Meningitis
Mice
Molecular Sequence Data
Monocytes
MyD88 protein
Myeloid Differentiation Factor 88 - immunology
Neonates
phagocytosis
Ribonucleic acid
RNA
RNA, Bacterial - immunology
RNA, Bacterial - physiology
Scientific Report
Scientific Reports
Sensors
Sepsis
signal transduction
Streptococcus
Streptococcus agalactiae - physiology
Streptococcus infections
Toll-like receptors
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Summary:Group B streptococcus (GBS) is a leading cause of both neonatal sepsis and meningitis, two diseases that are characterized by inflammation. However, the manner in which GBS organisms are recognized by monocytes and macrophages is poorly understood. In this study, we report that the recognition of GBS and other Gram‐positive bacteria by macrophages and monocytes relies on bacterial single‐stranded RNA (ssRNA). ssRNA interacts with a signalling complex, which comprises the Toll‐like receptor adaptors MyD88 and UNC‐93B, but not the established MyD88‐dependent ssRNA sensors. The role of ssRNA in the recognition of Gram‐positive bacteria—leading to the induction of inflammatory cytokines—has potential implications for sepsis pathogenesis, diagnosis and treatment. Recognition of streptococci and other Gram‐positive bacteria by macrophages and monocytes is shown here to rely on bacterial ssRNA. SsRNA interacts with a signaling complex, which comprises the TLR adapters MyD88 and UNC‐93B, but not the established MyD88‐dependent ssRNA sensors.
ISSN:1469-221X
1469-3178
DOI:10.1038/embor.2010.189