Experimentally Approaching the ICU : Monitoring Outcome-Based Responses in the Two-Hit Mouse Model of Posttraumatic Sepsis

To simulate and monitor the evolution of posttraumatic sepsis in mice, we combined a two-hit model of trauma/hemorrhage (TH) followed by polymicrobial sepsis with repetitive blood sampling. Anesthetized mice underwent femur fracture/sublethal hemorrhage and cecal ligation and puncture (CLP) 48 h lat...

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Bibliographic Details
Published in:BioMed research international 2011-01, Vol.2011 (2011), p.1-12
Main Authors: Drechsler, Susanne, Weixelbaumer, Katrin M., Redl, Heinz, van Griensven, Martijn, Bahrami, Soheyl, Osuchowski, Marcin F.
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Blood Cell Count
Blood Glucose - metabolism
Care and treatment
Disease Models, Animal
Epidemiology
Female
Health aspects
Hemorrhage
Intensive Care Units
Management
Methodology Report
Mice
Monitoring, Physiologic
Mortality
Organ Specificity
Risk factors
Rodents
Sepsis
Sepsis - blood
Sepsis - etiology
Sepsis - physiopathology
Studies
Survival Analysis
Trauma
Treatment Outcome
Wounds and Injuries - blood
Wounds and Injuries - complications
Wounds and Injuries - physiopathology
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Summary:To simulate and monitor the evolution of posttraumatic sepsis in mice, we combined a two-hit model of trauma/hemorrhage (TH) followed by polymicrobial sepsis with repetitive blood sampling. Anesthetized mice underwent femur fracture/sublethal hemorrhage and cecal ligation and puncture (CLP) 48 h later. To monitor outcome-dependent changes in circulating cells/biomarkers, mice were sampled daily (facial vein) for 7 days and retrospectively divided into either dead (DIE) or surviving (SUR) by post-CLP day 7. Prior to CLP, AST was 3-fold higher in DIE, while all other post-TH changes were similar between groups. There was a significant post-CLP intergroup separation. In SUR, RBC and Hb were lower, platelets and neutrophils higher, and lymphocytes mixed compared to DIE. In DIE, all organ function markers except glucose (decrease) were few folds higher compared to SUR. In summary, the combination of daily monitoring with an adequate two-hit model simulates the ICU setting, allows insight into outcome-based responses, and can identify biomarkers indicative of death in the acute posttraumatic sepsis in mice.
ISSN:1110-7243
2314-6133
1110-7251
2314-6141
DOI:10.1155/2011/357926