Primary structure and cellular localization of callinectin, an antimicrobial peptide from the blue crab

We report the complete amino acid sequence of callinectin, a 32 amino acid, proline-, arginine-rich antimicrobial peptide (AMP) with four cysteines and having the sequence WNSNRRFRVGRPPVVGRPGCVCFRAPCPCSNY-amide. The primary structure of callinectin is highly similar to arasins, AMPs recently identif...

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Bibliographic Details
Published in:Developmental and comparative immunology 2011-04, Vol.35 (4), p.409-415
Main Authors: Noga, Edward J., Stone, Kathryn L., Wood, Abbey, Gordon, William L., Robinette, David
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Animals
antibodies
Antimicrobial Cationic Peptides - chemistry
Antimicrobial peptide
antimicrobial peptides
Arginine
Brachyura - chemistry
Brachyura - immunology
Callinectes sapidus
crabs
Cystine
cytoplasmic granules
Decapoda
granules
hemocytes
Hyas araneus
Hydroxylated tryptophan
isomers
microscopy
Molecular Sequence Data
peptides
Proline
rabbits
Sequence Alignment
Thomisidae
tryptophan
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Summary:We report the complete amino acid sequence of callinectin, a 32 amino acid, proline-, arginine-rich antimicrobial peptide (AMP) with four cysteines and having the sequence WNSNRRFRVGRPPVVGRPGCVCFRAPCPCSNY-amide. The primary structure of callinectin is highly similar to arasins, AMPs recently identified in the small spider crab ( Hyas araneus). Callinectin exists in three isomers that vary in the functional group on the tryptophan (W) residue. The most prevalent isomer had a hydroxy-N-formylkynurenine group, while the other two isomers had either N-formylkynurenine or hydroxy-tryptophan. Using a sequence highly similar to native callinectin, we chemically synthesized a peptide which we called callinectin-like peptide (CLP). Via immuno-electron microscopy, affinity-purified rabbit antibodies raised to CLP successfully localized the site of callinectin in blue crab hemocytes to the large electron-dense granules that are found primarily in large granule hemocytes.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2010.11.015