Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and β-NGF genes

A novel in situ hybridization technique is described. This non-radioactive technique combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. The procedure, which employs biotin...

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Bibliographic Details
Published in:Nucleic acids research 1987-06, Vol.15 (12), p.4761-4770
Main Authors: GARSON, J. A, VAN DEN BERGHE, J. A, KEMSHEAD, J. T
Format: Article
Language:English
Subjects:
Alkaline Phosphatase
Azure Stains
Bacterial Proteins
Base Sequence
Biological and medical sciences
Biotin
Chromosome Banding - methods
Chromosome Mapping - methods
DNA, Recombinant
Fundamental and applied biological sciences. Psychology
Genes
Genes. Genome
Humans
Molecular and cellular biology
Molecular genetics
Nerve Growth Factors - genetics
Nucleic Acid Hybridization
Oncogenes
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-myc
Streptavidin
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Summary:A novel in situ hybridization technique is described. This non-radioactive technique combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. The procedure, which employs biotin labelled DNA probes and a streptavidin-alkaline phosphatase based detection system, is compatible with pre G-banding and can be performed on archival material. Unique sequences as small as 1 Kb are detectable. Using this technique, we have mapped the N-myc oncogene and the gene for beta-Nerve Growth Factor to 2p24 and 1p13 respectively.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.12.4761