Synthesis of a Trimeric gp120 Epitope Mimic Conjugated to a T-Helper Peptide To Improve Antigenicity

A fully synthetic trivalent mimotope of gp120 conjugated to pan allelic HLA DR binding epitope was prepared using solid-phase peptide synthesis and optimized copper-catalyzed azide−alkyne cycloaddition. The methodology efficiently provides chemically uniform heteromultimeric peptide constructs with...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2011-03, Vol.133 (10), p.3230-3233
Main Authors: Schellinger, Joan G, Danan-Leon, Lieza M, Hoch, Jessica A, Kassa, Aemro, Srivastava, Indresh, Davis, David, Gervay-Hague, Jacquelyn
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Epitopes, T-Lymphocyte - chemistry
Epitopes, T-Lymphocyte - immunology
HIV Envelope Protein gp120 - chemical synthesis
HIV Envelope Protein gp120 - chemistry
HIV Envelope Protein gp120 - immunology
HLA-DR Antigens - chemistry
HLA-DR Antigens - immunology
Immunodominant Epitopes - chemistry
Immunodominant Epitopes - immunology
Malaria Vaccines - chemistry
Molecular Sequence Data
Peptides - chemical synthesis
Peptides - chemistry
Peptides - immunology
T-Lymphocytes, Helper-Inducer - immunology
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Summary:A fully synthetic trivalent mimotope of gp120 conjugated to pan allelic HLA DR binding epitope was prepared using solid-phase peptide synthesis and optimized copper-catalyzed azide−alkyne cycloaddition. The methodology efficiently provides chemically uniform heteromultimeric peptide constructs with enhanced binding, avidity, and specificity toward an established HIV-neutralizing human antibody, MAb b12. The versatile synthetic strategy serves as a powerful platform for the development of synthetic peptides as potential HIV-1 vaccine candidates.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja1083915