Hybridization of microsatellites to RAPD: a new source of polymorphic markers

Genetic analyses of plant and animal populations and species for taxonomic, evolutionary and ecological studies tremendously benefited from the development of various molecular marker techniques which reveal polymorphisms at the DNA level. In general, DNA-based markers have important advantages such...

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Bibliographic Details
Published in:Nucleic acids research 1995-09, Vol.23 (18), p.3798-3799
Main Authors: Richardson, T., Cato, S., Ramser, J., Kahl, G., Weising, K.
Format: Article
Language:English
Subjects:
Ascomycota - genetics
Base Sequence
Dinucleotide Repeats
DNA Fingerprinting - methods
DNA Primers
DNA Probes
Genetic Markers
Microsatellite Repeats - genetics
Molecular Sequence Data
Nucleic Acid Hybridization
Plants - genetics
Polymorphism, Genetic
Random Amplified Polymorphic DNA Technique
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Summary:Genetic analyses of plant and animal populations and species for taxonomic, evolutionary and ecological studies tremendously benefited from the development of various molecular marker techniques which reveal polymorphisms at the DNA level. In general, DNA-based markers have important advantages such as selective neutrality, abundance, high variability and independence of environmental effects. Among the most widely applied techniques in plants are genomic RFLP analysis, multilocus DNA fingerprinting with mini- or microsatellite complementary probes, PCR amplification of individual microsatellite loci and PCR with arbitrary or semispecific primers that amplify anonymous regions of the genome. RFLPs and PCR-amplified microsatellites are locus-specific, codominant markers which proved highly useful for population genetic studies and the establishment of high density linkage maps. However, such locus-specific approaches require molecular cloning procedures whereas multilocus fingerprinting and anonymous PCR strategies do not. Therefore, the latter techniques are preferred tools to study species where little or no DNA sequence information is available. Here we report a new method which combines arbitrarily or semispecifically primed PCR with microsatellite hybridization to produce several independent and polymorphic genetic fingerprints per electrophoretic gel. No prior sequence information is needed. This novel method which we call RAMPO (random amplified microsatellite polymorphisms) is generally applicable for plants, and most probably for animals and humans as well.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/23.18.3798