Mouse neutrophils are professional antigen-presenting cells programmed to instruct Th1 and Th17 T-cell differentiation

Neutrophils play a major role in the innate immune system and are normally considered to be short-lived effector cells that exert anti-microbial activity and sometimes immunopathology. Here, we show that these cells possess an additional function as professional antigen-presenting cells capable of p...

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Bibliographic Details
Published in:International immunology 2011-05, Vol.23 (5), p.317-326
Main Authors: Abi Abdallah, Delbert S, Egan, Charlotte E, Butcher, Barbara A, Denkers, Eric Y
Format: Article
Language:English
Subjects:
Animals
Antigen-Presenting Cells - immunology
Antigens - immunology
Cell Differentiation - immunology
Cell Line
Female
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Neutrophils - immunology
Ovalbumin - immunology
Th1 Cells - cytology
Th1 Cells - immunology
Th17 Cells - cytology
Th17 Cells - immunology
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Summary:Neutrophils play a major role in the innate immune system and are normally considered to be short-lived effector cells that exert anti-microbial activity and sometimes immunopathology. Here, we show that these cells possess an additional function as professional antigen-presenting cells capable of priming a T(h)1- and T(h)17-acquired immune response. Using flow cytometry, fluorescence microscopy and western blotting, we show that mouse neutrophils express MHC class II and co-stimulatory molecules CD80 and CD86 after T-cell co-incubation. Neutrophils pulsed with ovalbumin (OVA) process and present peptide antigen to OVA-specific T cells in an MHC class II-dependent manner. Importantly, we demonstrate that neutrophils can prime antigen-specific T(h)1 and T(h)17 immune responses even without the addition of exogenous cytokines to cell cultures.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/dxr007