Structural organization of the Amy locus In seven strains of Drosophila melanogaster

• Published in: Nucleic acids research 1986-07, Vol.14 (13), p.5337-5352
• Main Authors: Gemmill, R.M., Schwartz, P.E., Doane, W.W.
• Format: Article
• Language: English
• Subjects: alpha-Amylases - genetics; Animals; Biological and medical sciences; Chromosome Deletion; Chromosome Inversion; Cloning, Molecular; DNA Restriction Enzymes; Drosophila melanogaster; Drosophila melanogaster - genetics; Fundamental and applied biological sciences. Psychology; Genes. Genome; Isoenzymes - genetics; Molecular and cellular biology; Molecular genetics; Mutation; Species Specificity
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/14.13.5337

Restriction maps were made by Southern blot analysis of the Amy (α-amylase) region in 7 strain, of D melanogaster using endonucleesee SalI, XhoI end EcoRI. These were compared to the map of λDm65 which contain, the cloned region. Strains used produce either two amylase variant, a single variant, or no amylase, yet all 7 strain, carry two Amy genes are inverted repeats at the Amy locus. This and the orientation of the repeats resenbles the situation in λDm65 Moat restriction sites mapped are conserved but two strains contain a large insertion which differs in size and position between strains. A complex anocely, probably an inversion, exists at the locus in a null strain. Maps for our Amy strain and the λDm65 clone are identical, the DNA of each having bean derived from a Canton-S wild stock. Reatriction and genetic cap. of the Amy region were aligned and alleles assigned to the proxisal and distal genes, Amy-p and Amy-d.